中国中药杂志

2015, v.40(07) 1271-1273

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沙苑子及其伪品直立黄芪的显微鉴别研究
Study on microscopic identification of Astragalus complanatus and A.adsurgens seeds

李晓琳;邵爱娟;展晓日;韦颖;黄璐琦;
LI Xiao-lin;SHAO Ai-juan;ZHAN Xiao-ri;WEI Ying;HUANG Lu-qi;State Key Laboratory of Dao-di Herbs,National Resource Center for Chinese Materia Medica,Chinese Academy of Chinese Medical Sciences;Colloge of Life and Environment Science,Hangzhou Normal University;

摘要(Abstract):

直立黄芪种子与沙苑子在外观形态上极为相似,是市场上常见的沙苑子伪品之一,其外观性状鉴别以及荧光法、紫外分光光度法、薄层色谱法、高效液相色谱法和蛋白电泳法等鉴别方法均有报道,但显微鉴别的相关报道较少。该文对沙苑子正品(扁茎黄芪种子)及其伪品直立黄芪种子进行了外观性状及显微鉴别研究,结果表明沙苑子和直立黄芪种子在颜色、形状上略有差异,在种皮和种脐的显微结构、子叶细胞中脂肪油滴的分布上与直立黄芪种子有显著差异。因此,显微鉴别可区别沙苑子和直立黄芪种子,为沙苑子药材鉴别提供科学依据。
Astragalus adsurgens seed is one of the most common adulterants of Astragali Complanati Semen in the market,whose morphological characteristics are very similar with A.complanatus seeds(Astragali Complanati Semen).Many identification methods have been reported,such as morphological identification,fluorescence method,ultraviolet spectrophotometry,TLC,HPLC,protein electrophoresis and so on,but there's no much about microscopic identification.In the present study,the morphological characteristics and microscopic characteristics of these two seeds were investigated,which could provide scientific evidence for the identification and classification of Astragali Complanati Semen.Our results showed that these two seeds were slightly different in the color and the appearance,but significantly different in the microstructure of the seed coat and the hilum,and the distribution of fat droplets in the cotyledon cells.So these microscopic characteristics can be applied for the identification of Astragali Complanati Semen.

关键词(KeyWords): 沙苑子;直立黄芪;种子;显微鉴别
Astragali Complanati Semen;Astragalus adsurgens;seeds;microscopic identification

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81102757);; 中国中医科学院基本科研业务费自主选题项目(zz0808012)

作者(Author): 李晓琳;邵爱娟;展晓日;韦颖;黄璐琦;
LI Xiao-lin;SHAO Ai-juan;ZHAN Xiao-ri;WEI Ying;HUANG Lu-qi;State Key Laboratory of Dao-di Herbs,National Resource Center for Chinese Materia Medica,Chinese Academy of Chinese Medical Sciences;Colloge of Life and Environment Science,Hangzhou Normal University;

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