中国中药杂志

2006, (10) 800-804

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指纹图谱技术对贯叶连翘提取物制备工艺过程的评价
Evaluation of preparing process of extract of Hypericum perforatum using HPLC fingerprint analysis

王冬梅;刘朝燊;杨得坡;
WANG Dong-mei,LIU Chao-shen,YANG De-po(Laboratory of Pharmacognosy and Natural Medicinal Chemistry,School of Pharmaceutical Sciences,Sun Yat-sen University,Guangzhou 510080,China)

摘要(Abstract):

目的:建立贯叶连翘提取物(含金丝桃素类和黄酮类化合物)的指纹图谱分析方法,并对提取物制备工艺的有效性、稳定性以及抗氧化剂、药材采收部位对提取物质量的影响进行综合评价。方法:采用HPLC-UV-MS法测定了药材与提取物的指纹图谱,并对主要色谱峰进行定性定量分析。结果:在提取物指纹图谱中标示了10个特征峰,鉴定了其中8个色谱峰所代表的化学成分。采用的提取物制备工艺稳定性良好,可有效地富集金丝桃素类和黄酮类化合物;抗氧化剂的使用对提取物的质量影响不大,但药材采收部位则对其有显著影响。结论:该指纹图谱分析方法可为提取物的质量控制以及制备工艺的评价提供依据。
Objective: To establish the quality control method of the extract enriched hypericins and flavonoids(HFEE) prepared from Hypericum perforatum by means of HPLC fingerprint analysis,and to evaluate the validity,stability of within-day and between-day of the preparing process of HFEE as well as the influence of antioxidant and the herbal source to the quality of HFEE.Method: HPLC-UV-MS was employed to the qualitative and quantitative analyses of HFEE.Result: Ten peaks on the HPLC fingerprint of HFEE were indicated,and eight compounds of them had been identified.With respect of the preparing process of HFEE,the validity and stability were significantly observed.There was almost no effect observed on the quality of HFEE whether or not adding the antioxidant during the process.However,different parts of the plant collected as the materials could significantly affect the quality of HFEE. Conclusion: The method established in the present study is convenient,reliable,and could be used for the quality control of the extract of H.perforatum and for the monitor and control of the preparing process of the extract.

关键词(KeyWords): 贯叶连翘提取物;提取制备工艺;HPLC指纹图谱
Hypericum perforatum;extraction;HPLC fingerprint

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(30470188);; 国家教育部高等学校骨干教师资助计划项目(2000)

作者(Author): 王冬梅;刘朝燊;杨得坡;
WANG Dong-mei,LIU Chao-shen,YANG De-po(Laboratory of Pharmacognosy and Natural Medicinal Chemistry,School of Pharmaceutical Sciences,Sun Yat-sen University,Guangzhou 510080,China)

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