袁青松;王绘;江维克;欧小宏;徐娇;王小爱;汪露;欧跃云;周涛;
YUAN Qing-song;WANG Hui;JIANG Wei-ke;OU Xiao-hong;XU Jiao;WANG Xiao-ai;WANG Lu;OU Yue-yun;ZHOU Tao;Guizhou University of Traditional Chinese Medicine;Guizhou Tiancheng Agricultural Science and Technology Development Co., Ltd.;

• 1:贵州中医药大学
• 2:贵州天成农业科技开发有限公司

摘要(Abstract)：

为创制天麻种子材料用于开展天麻有性繁殖分子机制研究奠定基础。该文对红天麻和乌天麻在变温(室温)和恒温(22℃)下抽薹后的花、蒴果、种子、种胚等13个性状进行分析,结果发现,恒温条件可以延长天麻抽薹持续时间,且能增加花朵数量,而天麻品种仅影响抽薹持续时间,对花朵数量无影响,并且红天麻比乌天麻更易抽薹;品种是影响天麻蒴果裂果主要的因素,温度是影响蒴果数和蒴果直径主要的因素,而恒温比室温更利于天麻蒴果的形成;天麻品种在种子的外观上无明显差异,而恒温条件下培育的天麻种子种胚比室温条件下培育的种子更加饱满;温度和品种对天麻种子外观的影响较小,而对种子的种胚的影响较大,恒温培育更利于天麻种子种胚的形成,红天麻比乌天麻种子更易形成种胚。该研究表明,乌天麻和红天麻种子发育可直接选择22℃恒温进行抽薹生产。
The study is aimed to create seed materials and dissect the molecular mechanism of sexual propagation of Gastrodia elata. In this research, thirteen characteristics of flowers, flower stem, fruits, seeds and embryo of G.elata f. glauca and G.elata f. elata after bolting at room temperature(RT) and constant temperature(CT, 22 ℃) were determined. It was found that the constant temperature condition could prolong the bolting duration of G.elata and increased the number of flowers, while the variety of G.elata only affected the bolting duration, but had no effect on the number of flowers, and the G.elata f. elata was more likely to bolting than the G.elata f. glauca. The variety of G.elata was the main factor affecting the time of dehiscent fruit of G.elata, the temperature was the main factor affecting the fruits number and fruits diameter, and the constant temperature was more conducive to the fruits shape of G.elata than the room temperature. There was no significant difference in seed phenotype of G.elata varieties, but the seed embryo of G.elata seeds cultivated at constant temperature was fuller than that of G.elata cultivated at room temperature, and temperature had less influence on the seed phenotype of G.elata. But it was interesting to find that temperature and varieties had greater influence on the seed embryo of G.elata, constant temperature cultivation was more conducive to the formation of seed embryo of G.elata, and more the seed embryo of G.elata f. elata was easier to form than the seed embryo of G.elata f. glauca. However, the development of seeds and embryos of G.elata was significantly affected, and the development of seeds and embryos of G.elata f. glauca was more sensitive to temperature than G.elata f. elata. The research suggested that it is advisable for G.elata to produce seed materials by bolting at constant temperature(22 ℃).

关键词(KeyWords)： 天麻;种子发育;温度;抽薹
Gastrodia elata;seed development;temperature;bolting

Abstract:

Keywords:

基金项目(Foundation): 中央本级重大增减支项目(2060302);; 国家重点研发计划中医药现代化研究专项(2017YFC1700703);; 国家自然科学基金项目(81960694);; 贵州省科技创新人才团队项目(黔科合平台人才[2019]5611);; 贵州省高层次创新型人才项目(黔科合平台人才[2018]5638);; 贵州省教育厅创新群体重大研究项目(黔教合KY字[2018]022);; 贵州省科技计划项目(黔科合支撑[2018]2764);; 贵州中医药大学博士启动基金项目([2019]55)

作者(Author): 袁青松;王绘;江维克;欧小宏;徐娇;王小爱;汪露;欧跃云;周涛;
YUAN Qing-song;WANG Hui;JIANG Wei-ke;OU Xiao-hong;XU Jiao;WANG Xiao-ai;WANG Lu;OU Yue-yun;ZHOU Tao;Guizhou University of Traditional Chinese Medicine;Guizhou Tiancheng Agricultural Science and Technology Development Co., Ltd.;

Email:

DOI: 10.19540/j.cnki.cjcmm.20191204.109

参考文献(References)：

• [1] OJEMANN L M,NELSON W L,SHIN D S,et al.Tian ma,an ancient Chinese herb,offers new options for the treatment of epilepsy and other conditions[J].Epilepsy Behav,2006,8:376.
• [2] 杨文权.天麻种质资源的研究[D].杨凌:西北农林科技大学,2005.
• [3] 邹宁,吴电云,柏新富,等.天麻细胞工程技术及其原理研究[J].安徽农业科学,2009,37(11):4980.
• [4] 张志龙,郜玉钢,臧埔,等.天麻素、对羟基苯甲醇对中枢神经系统作用机制研究进展[J].中国中药杂志,2020,45(2):312.
• [5] 康传志,杨婉珍,周利,等.不同产地和变型天麻药材的质量及其硫磺熏蒸前后的变化[J].中国中药杂志,2018,43(2):254.
• [6] 李梅,刘帆,陈艳,等.基于大鼠在体循环肠灌流模型研究不同粒径天麻粉的肠吸收动力学[J].中国中药杂志,2019,doi:10.19540/j.cnki.cjcmm.20191018.201.
• [7] CHEN C,FU Y H,LI M H,et al.Nuclear magnetic resonance-based metabolomics approach to evaluate preventive and therapeutic effects of Gastrodia elata Blume on chronic atrophic gastritis[J].J Pharmaceut Biomed,2019,164:231.
• [8] 周元.天麻生物学特性研究[D].杨凌:西北农林科技大学,2005.
• [9] 王绍柏,余昌俊,刘湘林,等.天麻花期调控试验[J].中药材,1995,18(11):543.
• [10] 石子为,马聪吉,康传志,等.基于空间分析的昭通天麻生态适宜性区划研究[J].中国中药杂志,2016,41(17):3155.
• [11] 徐锦堂,冉砚珠,王孝文,等.天麻有性繁殖方法的研究[J].药学学报,1980,15(2):100.
• [12] 徐锦堂,冉砚珠,郭顺星.天麻生活史的研究[J].中国医学科学院学报,1989,11(4):237.
• [13] 陈英,韩义,王玉玫,等.西洋参种胚发育的温度要求[J].中草药,1987,18(5):33.
• [14] 廖云娇,李雪,董学会.不同变温层积过程中东北红豆杉种子生理生化特性和胚形态的变化[J].中国农业大学学报,2010,15(1):39.
• [15] YUAN Y,JIN X,LIU J,et al.The Gastrodia elata genome provides insights into plant adaptation to heterotrophy[J].Nat Commun,2018,9:1615
• [16] ZENG X,LI Y,LING H,et al.Transcriptomic analyses reveal clathrin-mediated endocytosis involved in symbiotic seed germination of Gastrodia elata[J].Bot Stud,2017,58:31
• [17] 程立君,陈玉惠,徐畅,等.昭通天麻种子萌发菌的筛选[J].安徽农业科学,2009,37(28):13600.