古丽娜.沙比尔;崔亚君;郭慧;王炜;果德安;
SABIR Gulnar~(1,2),CUI Ya-jun~1,GUO Hui~1,WANG Wei~1,GUO De-an~1(1.School of Pharmaceutical Sciences,Peking University,Beijing 100083,China;2.Xingjiang Institute of Chinese Material Medical and Ethical,Urumqi 830002,China)

• 1:北京大学药学院
• 2:北京大学药学院
• 3:北京大学药学院
• 4:北京大学药学院

摘要(Abstract)：

目的:建立羌活药材的色谱指纹图谱,为评价羌活药材的质量提供依据。方法:采用Phenom enex Lu-na C18(2)(4.6 mm×250 mm,5μm)色谱柱,乙腈(A)-0.3%醋酸水(B)为流动相梯度洗脱,流速1 mL.m in-1,检测波长330 nm,柱温30℃,洗脱时间70 m in。结果:得到分离度较好的羌活HPLC-UV色谱指纹图谱,标示了7个共有峰。并采用对照品比对和HPLC-MS技术对其部分色谱峰进行了定性鉴别。结论:色谱指纹图谱分析方法为全面控制羌活药材的质量提供了依据。
Objective: To establish the chromatographic fingerprints for the identification and quality evaluation of Rhizoma et Radix Notopterygii.Method: Separation was performed on Phenomenex Luna C_(18)(2)(4.6 mm×250 mm,5 μm) column,with mobile phase consisting of acetonitrile(A) and water containing 0.3% acetic acid(B) with gradient elution at the flow rate of 1 mL· min~(-1).The UV detection wavelength was 330 nm,column temperature was 30 ℃,and the analysis time was 70 min.Result: A satisfactory fingerprint was obtained by HPLC method,and qualitative analysis of some of the peaks were carried on by HPLC/MS approach.Conclusion: The established method is simple and rapid to identity and differentiate samples of Nhizoma et Radix Notopterygii collected from different sources and might be used as a quality control method for Rhizoma et Radix Notopterygii.

关键词(KeyWords)： 羌活;HPLC-UV-MS;色谱指纹图谱
Rhizoma et Radix Notopterygii;HPLC-UV-MS;fingerprint

Abstract:

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基金项目(Foundation):

作者(Author): 古丽娜.沙比尔;崔亚君;郭慧;王炜;果德安;
SABIR Gulnar~(1,2),CUI Ya-jun~1,GUO Hui~1,WANG Wei~1,GUO De-an~1(1.School of Pharmaceutical Sciences,Peking University,Beijing 100083,China;2.Xingjiang Institute of Chinese Material Medical and Ethical,Urumqi 830002,China)

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参考文献(References)：

• [1]江苏新医学院.中药大辞典[M].上册.上海:上海科学技术出版社,1986:1172.
• [2]李云霞,高春华,沙明.中药羌活化学成分及药理作用研究进展[J].辽宁中医学院学报,2004,6(1):22.
• [3]中国药典[S].一部.2005:127.
• [4]高广慧,邓捷圆,王秀敏,等.中药材羌活质量控制的气相色谱指纹图谱[J].沈阳药科大学学报,2004,21(6):438.
• [5]杨秀伟,严仲铠,顾哲明.羌活化学成分的研究[J].中草药,1993,24(10):507.