肖亚平;曾杰;焦琳娜;徐晓玉;
XIAO Ya-ping;ZENG Jie;JIAO Lin-na;XU Xiao-yu;College of Pharmaceutical Sciences & Chinese Medicine,Southwest University;Chongqing Key Laboratory of New Drug Screening from Traditional Chinese Medicine;Pharmacology of Chinese Materia Medica-the Key Discipline Constructed by the State Administration of Traditional Chinese Medicine;

• 1:西南大学药学院&中医药学院
• 2:重庆市高校中药新药筛选重点实验室
• 3:国家中医药管理局中药药理学重点建设学科

摘要(Abstract)：

有关补肾中药对于骨质疏松症的治疗及其信号通路调节作用的研究较多,但缺乏系统性的归纳总结。该文综合整理和分析了近10年来淫羊藿、骨碎补、蛇床子、杜仲、补骨脂、续断等经典补肾中药对骨质疏松症的治疗作用及其信号通路的调节作用。根据现有研究结果得出如下结论:(1)补肾中药主要通过BMP-Smads、Wnt/β-catenin、MAPK、PI3K/AKT等信号通路,促进成骨细胞的骨形成作用;通过OPG/RANKL/RANK、雌激素、CTSK等信号通路抑制破骨细胞的骨吸收作用,达到治疗骨质疏松症的目的。淫羊藿、骨碎补、蛇床子、补骨脂可通过上调BMP-Smads、Wnt/β-catenin信号通路中BMP-2、Smad1/5/8、Wnt3a及β-catenin等关键蛋白及基因的表达,促进成骨细胞的骨形成;淫羊藿、骨碎补、蛇床子、杜仲、补骨脂、续断通过介导OPG/RANKL/RANK信号通路,上调OPG和OPG/RANKL的表达,抑制破骨细胞的骨吸收。(2)补肾中药能通过多种途径防治骨质疏松症:淫羊藿中的淫羊藿苷、骨碎补中的柚皮苷、蛇床子中的蛇床子素、补骨脂中的补骨脂素既可以通过调控BMP-Smads、Wnt/β-catenin等信号通路促进骨形成,又可以活化OPG/RANKL/RANK、CTSK等信号通路抑制骨吸收。(3)补肾中药对防治骨质疏松症的信号通路的相互联系(crosstalk)和整体动物实验的研究有待进一步深入,有待阐明其多靶点和多途径的作用机制与综合调控作用。
The treatment effect and signaling pathway regulation effects of kidney-tonifying traditional Chinese medicine on osteoporosis have been widely studied,but there is no systematic summary currently.This review comprehensively collected and analyzed the traditional Chinese medicines on the treatment and signaling pathway regulation of osteoporosis in recent ten years,such as Epimedii Folium,Drynariae Rhizoma,Cnidii Fructus,Eucommiae Cortex,Psoraleae Fructus and Dipsaci Radix.Based on the existing findings,the following conclusions were obtained:(1) kidney-tonifying traditional Chinese medicine treated osteoporosis mainly through BMPSmads,Wnt/β-catenin,MAPK,PI3K/AKT signaling pathway to promote osteoblast bone formation and through OPG/RANKL/RANK,estrogen,CTSK signaling pathway to inhibit osteoclasts of bone resorption.Epimedii Folium,Drynariae Rhizoma,Cnidii Fructus and Psoraleae Fructus up-regulated the expression of key proteins and genes of BMP-Smads and Wnt/β-catenin signaling pathways to promote bone formation.Epimedii Folium,Drynariae Rhizoma,Cnidii Fructus,Eucommiae Cortex,Psoraleae Fructus and Dipsaci Radix inhibited the bone resorption by mediating the OPG/RANKL/RANK signaling pathway.(2) Kidney-tonifying traditional Chinese medicine prevented and treated osteoporosis through a variety of ways:icariin in Epimedii Folium,naringin in Drynariae Rhizoma,osthole in Cnidii Fructus and psoralen in Psoraleae Fructus can regulate BMP-Smads,Wnt/β-catenin signaling pathway to promote bone formation,but also activate OPG/RANKL/RANK,CTSK and other signaling pathways to inhibit bone resorption.(3)The crosstalk of the signaling pathways and the animal experiments of the traditional Chinese medicine on the prevention and treatment of osteoporosis as well as their multi-target mechanism and comprehensive regulation need further clarification.

关键词(KeyWords)： 补肾中药;骨质疏松症;信号通路;骨代谢;成骨细胞;破骨细胞
kidney-tonifying traditional Chinese medicine;osteoporosis;signaling pathways;bone metabolism;osteoblast;osteoclast

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金面上项目(81473549);; 教育部中央高校基本科研业务费项目(XDJK2017D158)

作者(Authors): 肖亚平;曾杰;焦琳娜;徐晓玉;
XIAO Ya-ping;ZENG Jie;JIAO Lin-na;XU Xiao-yu;College of Pharmaceutical Sciences & Chinese Medicine,Southwest University;Chongqing Key Laboratory of New Drug Screening from Traditional Chinese Medicine;Pharmacology of Chinese Materia Medica-the Key Discipline Constructed by the State Administration of Traditional Chinese Medicine;

DOI: 10.19540/j.cnki.cjcmm.20171106.002

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