中国中药杂志

2020, v.45(13) 3183-3190

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基于道地产区蒙古黄芪的质量差异性分析
Analysis of quality difference based on Astragalus membranaceus var. mongholicus in genuine region

赵晨光;李存玉;杨珊;许启龙;郑云枫;王玉富;李红阳;彭国平;
ZHAO Chen-guang;LI Cun-yu;YANG Shan;XU Qi-long;ZHENG Yun-feng;WANG Yu-fu;LI Hong-yang;PENG Guo-ping;College of Pharmacy, Nanjing University of Chinese Medicine;Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization;Ningxia Industry Association of Traditional Chinese Medicine;

摘要(Abstract):

采用HPLC-ELSD建立黄芪指纹图谱,分析内蒙古、宁夏、甘肃道地产区黄芪仿野生程度。以野生黄芪为对照,通过色谱峰指认,并结合相似度评价、聚类分析、主成分分析及正交偏最小二乘法判别分析探讨道地产区黄芪的质量差异。建立了不同道地产区黄芪药材HPLC指纹图谱;质谱定性分析指认出18个成分;相似度评价显示32批黄芪样品相似度为0.688~0.993,其中,山西产区样品相似度在0.688~0.993,内蒙古产区样品相似度在0.835~0.989,宁夏产区样品相似度在0.934~0.988,甘肃产区除25号样品外,其余样品相似度在0.729~0.876;聚类分析显示除样品11和25外,黄芪样品可按产区聚为4类;主成分分析结果表明32批黄芪样品可按品质和产地聚类且内蒙古产区黄芪的仿野生程度最高;正交偏最小二乘法判别分析筛选出野生、家种黄芪差异性的6个成分为丙二酰基黄芪皂苷Ⅰ、黄芪皂苷Ⅰ、丙二酰基毛蕊异黄酮苷、毛蕊异黄酮苷、丙二酰基芒柄花苷、丙二酰基黄芪紫檀烷苷。所建立的方法可用于分析黄芪的产地和种植环境差异,可为野生黄芪资源的替代和保护及黄芪的种植、加工、生产提供参考。
This work is to establish the fingerprint of Astragalus membranaceus var. mongholicus by HPLC-ELSD method, and to analyze the simulated wildness degree of A. membranaceus var. mongholicus in the genuine region of Inner Mongolia, Ningxia and Gansu. Compared with wild A. membranaceus var. mongholicus, the quality differences of A. membranaceus var. mongholicus in the genuine region were analyzed by identification of chromatographic peaks and similarity evaluation, cluster analysis(CA), principal components analysis(PCA) and orthogonal partial least squares discriminant analysis(OPLS-DA). HPLC fingerprints of A. membranaceus var. mongholicus in different genuine regions are established. The qualitative analysis of mass spectrometry identified 18 components. The similarity evaluation shows that the similarity of 32 batches of A. membranaceus var. mongholicus samples was 0.688-0.993. Among them, the similarity of samples in Shanxi, Inner Mongolia, Ningxia is 0.688-0.993, 0.835-0.989, 0.934-0.988, respectively and the similarity of samples in Gansu is 0.729-0.876 except No. 25 sample. The results of CA show that the samples of A. membranaceus var. mongholicus can be grouped into four categories according to the production area except the No. 11 and No. 25 samples. The results of PCA indicate that 32 batches of A. membranaceus var. mongholicus samples can be clustered according to quality and origin, and the quality of A. membranaceus var. mongholicus in Inner Mongolia is the closest to the wild breed. The results of OPLS-DA indicate that there are six components that can distinguish the wild and domestic A. membranaceus var. mongholicus, which are malonylastragaloside Ⅰ, astragaloside Ⅰ, calycosin-7-O-β-D-glycoside-6″-O-malonate, calycosin-7-O-β-D-glycoside, formononetin-7-O-β-D-glycoside-6″-O-malonate, and astrapterocarpan-3-O-β-D-glycoside-6″-O-malonate. The established method can be used to analyze differences between A. membranaceus var. mongholicus origin and planting environment, and can provide references for the protection and replacement of wild A. membranaceus var. mongholicus resources, and the cultivation, processing and production of A. membranaceus var. mongholicus.

关键词(KeyWords): 蒙古黄芪;指纹图谱;聚类分析;主成分分析;正交偏最小二乘法判别分析
Astragalus membranaceus var.mongholicus;fingerprint;cluster analysis;principal component analysis;orthogonal partial least squares discriminant analysis

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81603307);; 校企合作项目(经典名方当归补血汤)

作者(Author): 赵晨光;李存玉;杨珊;许启龙;郑云枫;王玉富;李红阳;彭国平;
ZHAO Chen-guang;LI Cun-yu;YANG Shan;XU Qi-long;ZHENG Yun-feng;WANG Yu-fu;LI Hong-yang;PENG Guo-ping;College of Pharmacy, Nanjing University of Chinese Medicine;Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization;Ningxia Industry Association of Traditional Chinese Medicine;

Email:

DOI: 10.19540/j.cnki.cjcmm.20200424.204

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