中国中药杂志

2017, v.42(09) 1717-1721

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基于微流控芯片技术的荆芥不同药用部位抗肺肿瘤药效关系研究
Study on relationship between efficacy against lung cancer and different parts of Schizonepeta tenuifolia based on microfluidic chip technology

樊佳新;包永睿;孟宪生;王帅;李天娇;
FAN Jia-xin;BAO Yong-rui;MENG Xian-sheng;WANG Shuai;LI Tian-jiao;College of Pharmacy,Liaoning University of Traditional Chinese Medicine;Liaoning Province Multi-Component Chinese Medicine Engineering Technology Research Center;Liaoning Province Modern Traditional Chinese Medicine Research and Engineering Laboratory;

摘要(Abstract):

为了进一步明确荆芥不同药用部位的合理使用,本文基于微流控芯片技术开展荆芥药材不同药用部位的体外抗肺肿瘤药效学研究,HPLC色谱法建立不同药用部位荆芥药材的指纹图谱,灰色关联度软件对其将药效指标与指纹图谱的相关信息进行谱效关系分析,HPLC-Q-TOF-MS的技术手段对其关联程度较大的化学成分进行快速分析与鉴定。体外药效结果显示,不同的入药部位具有一定的差异性,诱导肺癌细胞凋亡坏死能力叶>花>根>茎;成分鉴定结果显示,第26,12,2,6,15号峰所代表的化合物依次为木犀草素、木犀草苷、迷迭香酸、咖啡酸、橙皮苷;第20,10号峰所代表的化合物可能依次为丹酚酸L、苯甲酸。该研究初步反映荆芥药材根部有纳入药用部位的趋势,并明确了荆芥药材中发挥抗肺肿瘤作用的主要化学组成,为荆芥药材以药理药效为导向的质量控制及不同药用部位的合理使用提供实验依据和实验方法。
In order to further clarify the rational use of different medicinal parts of Schizonepeta,microfluidic technology was used in this study to investigate the differences in drug efficacy against lung cancer in vitro. The ethanol extracts were examined with HPLC to establish their fingerprints in order to analyze the relationship between the spectrum and efficacy index through Grey Correlation software,and a rapid HPLC-Q-TOF/MS method was established. The result in vitro shows that the effect and components of different medicinal parts had a certain differences,and apoptosis and necrosis rate from big to small in turn is leaf,flower,root,stem. The chromatographic peaks of the 26,12,2,6 and 15 thare the luteolin,icynaroside,rosmarinic,caffeic acid,and hesperidin,while the 20 and10 thmay be dan phenolic acid L and benzoic acid. On the one hand,preliminary study reflects that the root of Schizonepeta tenuifolia may be developed into the medicinal parts in future. On the other hand,the major chemical composition of S. tenuifolia was found to have the anti-lung-tumor effects. This new method was established for the quality control and the rational use of different parts of traditional Chinese medicine.

关键词(KeyWords): 荆芥;肺癌;指纹图谱;微流控芯片;关联性分析
Schizonepeta tenuifolia;lung cancer;fingerprints;microfluidic chip;grey correlation degree

Abstract:

Keywords:

基金项目(Foundation): 国家“重大新药创制”科技重大专项(2013ZX09507005);; 辽宁省一般项目(L2014361,L201605)

作者(Author): 樊佳新;包永睿;孟宪生;王帅;李天娇;
FAN Jia-xin;BAO Yong-rui;MENG Xian-sheng;WANG Shuai;LI Tian-jiao;College of Pharmacy,Liaoning University of Traditional Chinese Medicine;Liaoning Province Multi-Component Chinese Medicine Engineering Technology Research Center;Liaoning Province Modern Traditional Chinese Medicine Research and Engineering Laboratory;

Email:

DOI: 10.19540/j.cnki.cjcmm.20170224.004

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