中国中药杂志

2011, v.36(22) 3083-3085

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南、北五味子RAPD-SCAR鉴别研究
Identification of Schisandra sphenanthera and S.chinensis by random amplified polymorphic DNA sequence characterized applied region

陈丽静;齐欣;王玉坤;张丽;郭志富;林景卫;宋宇宁;钟鸣;
CHEN Lijing1,QI Xin 1,WANG Yukun1,ZHANG Li1,GUO Zhifu1,LIN Jingwei1,SONG Yuning2,ZHONG Ming1(1.Bioscience and Technology Institute of Shenyang Agriculture University,Shenyang 110161,China; 2.Liaoning Institute of Traditional Chinese Medicine,Shenyang 110161,China)

摘要(Abstract):

目的:为准确鉴别南五味子和北五味子寻找一种新的鉴别手段。方法:采用RAPD-SCAR技术对南、北五味子基因组DNA进行PCR扩增。结果:100条RAPD引物中筛选出2条条带清楚、重复性好的RAPD引物能够区分南、北五味子,并将其转化为SCAR标记。结论:RAPD-SCAR技术是在分子水平上鉴别南、北五味子的一种行之有效的方法。
Objective: To establish a new method for the identification of Schisandra sphenanthera and S.chinensis.Method: Random amplified polymorphic DNA-Sequence characterized applied region(RAPD-SCAR) method was applied to screen primers.Result: Screening from 100 primers,only 2 random primers,which can be used to identify S.sphenanthera and S.chinensis accurately with a good reproducibility.It worked to fit them into sequence characterized applied region.Conclusion: RAPD-SCAR can be used to identify S.sphenanthera and S.chinensis accurately.

关键词(KeyWords): 南、北五味子;RAPD标记;SCAR标记;中药鉴定
Schisandra sphenanthera and S.chinensis;RAPD marker;SCAR marker;identification of herbal medicine

Abstract:

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基金项目(Foundation): 辽宁省自然基金博士启动基金项目(20081067);; 辽宁省自然科学基金项目(20072124)

作者(Author): 陈丽静;齐欣;王玉坤;张丽;郭志富;林景卫;宋宇宁;钟鸣;
CHEN Lijing1,QI Xin 1,WANG Yukun1,ZHANG Li1,GUO Zhifu1,LIN Jingwei1,SONG Yuning2,ZHONG Ming1(1.Bioscience and Technology Institute of Shenyang Agriculture University,Shenyang 110161,China; 2.Liaoning Institute of Traditional Chinese Medicine,Shenyang 110161,China)

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