游佳莉;王毓杰;张艺;杨洋;苏永文;范刚;
YOU Jia-li;WANG Yu-jie;ZHANG Yi;YANG Yang;SU Yong-wen;FAN Gang;College of Ethnic Medicine,Chengdu University of Traditional Chinese Medicine;

• 1:成都中医药大学民族医药学院

摘要(Abstract)：

通过对不同产地藏药沙棘膏的HPLC指纹图谱分析,为沙棘膏的品种鉴定和制备工艺的研究奠定基础。实验使用Agilent zorbax SB-C18(4.6 mm×250 mm,5μm)色谱柱,以乙腈-0.4%磷酸溶液为流动相梯度洗脱,流速1.0 m L·min-1,柱温30℃,检测波长360 nm。建立了15个批次沙棘膏的共有图谱,提取了7个色谱峰作为指纹图谱共有峰,结合对照品对其中的3个色谱峰进行了指认,分别为槲皮素、山柰素、异鼠李素;15批药材中第14批次与对照指纹图谱比较相似度小于0.9,其他批次的相似度均大于0.9。该研究首次采用HPLC建立藏药沙棘膏的指纹图谱,该方法具有良好的精密度、稳定性和重复性,为沙棘膏质量控制和评价提供依据。
This study established an HPLC fingerprint of Tibetan medicine Shaji Gao from different habitats and lay a foundation for Shaji Gao varieties identification and preparation process. The chromatographic condition was as follow: Agilent zorbax SB-C18( 4. 6 mm × 250 mm,5 μm) eluted with the mobile phases of acetonitrile and 0. 4% phosphoric acid water in gradient mode. The flow rate was 1. 0 m L·min- 1,and the detection wavelength was set at 360 nm. The fingerprints of 15 batches Shaji Gao were carried out by similarity comparation,7 chromatographic peaks were extracted as the common peaks of fingerprint,3 peaks were identified,which were quercetin,kaempferol and isorhamnetin. The similarity degrees of 14 batches of samples were above 0. 9 and 1 batch of samples was below 0. 9. This is the first established fingerprint of Shaji Gao by using HPLC. This method has good precision,stability and repeatability that it could provide basis for quality control and evaluation of Shaji Gao.

关键词(KeyWords)： 藏药;沙棘膏;HPLC;指纹图谱
Tibetan medicinal material;Shaji Gao;HPLC;Chromatographic fingerprint

Abstract:

Keywords:

基金项目(Foundation): 中国药典一部(2015年版)科研任务;; 四川省教育厅四川省省属高校科研创新团队建设计划项目(11TD004)

作者(Author): 游佳莉;王毓杰;张艺;杨洋;苏永文;范刚;
YOU Jia-li;WANG Yu-jie;ZHANG Yi;YANG Yang;SU Yong-wen;FAN Gang;College of Ethnic Medicine,Chengdu University of Traditional Chinese Medicine;

Email:

DOI:

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