中国中药杂志

2004, (07)

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菟箭合剂对糖尿病大鼠肾组织蛋白激酶C活性作用的研究
Effect of Tujian decoction on protein kinase C activity in renal cortex in diabetic rat

朴元林,梁晓春,杨楠,尹德海,左萍萍
PIAO Yuan-lin1, LIANG Xiao-chun1, YANG Nan2, YIN De-hai1, ZUO Ping-ping2 (1.Department of Traditional Chinese Medicine, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China; 2.Department of Pharmacology, Institute of Basical Medical Sciences, Peking Union Medical College,Chinese Academy of Medical Sciences, Beijing 100005, China)

摘要(Abstract):

目的 :观察菟箭合剂对实验性糖尿病肾病大鼠肾组织蛋白激酶C(PKC)活性、肾功能及肾脏结构的影响。方法 :单肾切除加链脲佐菌素 (STZ)腹腔注射制作糖尿病肾病动物模型。中药菟箭合剂 (2 0g·kg-1·d-1)和阳性对照药缬沙坦 (2 0mg·kg-1·d-1)干预治疗 12周 ,用氚标记的二丁酸佛波醇酯 ([3 H]PDBu)结合法测定大鼠肾组织PKC活性 ,检测 2 4h尿蛋白排出量 (Upro) ,肾脏常规染色光镜观察病理变化。结果 :12周时单肾糖尿病大鼠肾组织出现肾小球硬化病理改变 ,Upro明显升高 ,细胞膜PKC活性 (mPKC)明显升高 ,细胞浆PKC活性 (cPKC)明显降低 ,mPKC和cPKC比值 (M/C)明显升高。菟箭合剂和缬沙坦干预治疗显著降低Upro ,降低mPKC和M/C ,升高cPKC(P <0 .0 1) ,改善肾组织病理改变。结论 :菟箭合剂对单肾切除STZ糖尿病大鼠具有肾保护作用 ,推测部分地通过抑制肾组织PKC激活途径来达到的。
Objective: To investigate influence of administration of Tujian decoction (Chinese herbal medicine) on protein kinase C (PKC) activity,renal function and structure in diabetic rat kidney. Method: Experimental diabetic nephropathy model was induced by nephrectomy combined with streptozotocin (STZ) injection in sprague-dawley rat. Tujian decoction (20 g·kg -1·d -1) and Valsartan(20 mg·kg -1·d -1)were orally administrated respectively for 12 weeks. PKC activity was measured by [3H]phorbol 12,13-dibutyrate ([3H]PDBu) binding assay. 24 h urine protein excretion (Upro) and renal pathological changes were observed. Result: In 12th week, diabetic nephrectomized rats developed proteinuria, glomerulosclerosis, increased membrane PKC activity (mPKC), decreased cytosol PKC (cPKC), and increased ratio of mPKC and cPKC (M/C). Administration of Tujian decoction or Valsartan led to a reduction in proteinuria, structural injury, mPKC and M/C, and a recovery in cPKC. Conclusion: Tujian decoction possesses a renoprotective effect on diabetic nephrectomized rat, at least partially via the inhibition of PKC activation in renal cortex.

关键词(KeyWords): 蛋白激酶C;糖尿病肾病;菟箭合剂;缬沙坦
protein kinase C; diabetic nephropathy; Tujian decoction;valsartan

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作者(Author): 朴元林,梁晓春,杨楠,尹德海,左萍萍
PIAO Yuan-lin1, LIANG Xiao-chun1, YANG Nan2, YIN De-hai1, ZUO Ping-ping2 (1.Department of Traditional Chinese Medicine, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China; 2.Department of Pharmacology, Institute of Basical Medical Sciences, Peking Union Medical College,Chinese Academy of Medical Sciences, Beijing 100005, China)

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