中国中药杂志

2007, (18) 1844-1847

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四川道地药材泽泻指纹图谱的建立及其质量研究
Establishment of chromatographic fingerprint and quality assessment on Sichuan native medicinal plant Alisma plantago-aquatica

刘红昌;杨文钰;陈兴福;
LIU Hong-chang YANG Wen-yu CHEN Xing-fu College of Agronomy,Sichuan Agricultural University,Yaan 625014,China

摘要(Abstract):

目的:建立泽泻药材的指纹图谱,研究四川不同产区泽泻药材的质量,为科学评价及有效控制其质量提供参考。方法:采用梯度洗脱法进行色谱分离,使用"中药指纹图谱相似度计算软件"进行数据处理。结果:17批样品得到的色谱指纹图谱有26个共有峰,16和22号峰分别被鉴定为24-乙酰泽泻醇A和23-乙酰泽泻醇B。结论:泽泻药材的指纹图谱特征性及专属性强,可结合含量测定用于全面控制其质量,确保每批产品的均一性。
Objective:To establish chromatographic fingerprint of Sichuan native medicinal plant Alisma plantago-aquatica by RP-HPLC for the quality control.Method:The gradient elution mode was applied in chromatographic separation,and data were ana- lyzed by"Computer Aided Similarity Evaluation"software to compare the quality of A.plantago-aquatica samples from different habi- tats.Result:The HPLC chromatographic fingerprinting of A.plantago-aquatica with 26 characteristic peaks was established from 17 lots of A.plantago-aquatica samples,peak 16 and 22 were identified as 24-acetyl alisol A and 23-acetyl alisol B,respectively.Con- clusion:The chromatographic fingerprinting of A.plantago-aquatica with high specificity can be used to control its quality and assure lot to lot consistency.The RP-HPLC fingerprint method is repeatable,feasible in analysis of A.plantago-aquatica.

关键词(KeyWords): 泽泻;道地药材;指纹图谱
Alisma plantago-aquatica;native medicinal plant;chromatographic fingerprint

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作者(Author): 刘红昌;杨文钰;陈兴福;
LIU Hong-chang YANG Wen-yu CHEN Xing-fu College of Agronomy,Sichuan Agricultural University,Yaan 625014,China

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