张静娴;崔艳梅;
ZHANG Jing-xian;CUI Yan-mei;State Key Laboratory of Bioactive Substance and Function of Natural Medicines,Ministry of Education & Institute of Materia Medica,Chinese Academy of Medical Science and Peking Union Medical College;

• 1:中国医学科学院北京协和医学院药物研究所天然药物活性与功能国家重点实验室

摘要(Abstract)：

中药何首乌乙酸乙酯部位经硅胶柱色谱、ODS反相柱色谱、Sephadex LH-20凝胶过滤和高效液相制备等方法进行分离纯化,通过1D-NMR,2D-NMR和HR-ESI-MS等波谱学方法鉴定分得化合物的结构,共分离并鉴定了6个化合物,分别为2,3,5,4'-四羟基二苯乙烯-2-O-(2″-O-对羟基苯甲酰基)-β-D-葡萄糖苷(1),2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷(2),2,4,6,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷(3),大黄素-8-O-β-D-葡萄糖苷(4),大黄素甲醚-8-O-β-D-葡萄糖苷(5),大黄素-8-甲醚(6)。其中化合物1为新化合物。
Six compounds were isolated from the ethyl acetate extract of the tuberous root of Polygonum multiflorum by silical gel,ODS revsered-phase silica gel,Sephadex LH-20,and preparative HPLC. The structures were elucidated as column chromatography over: 2,3,5,4'-tetrahydroxystilbene-2-O-( 2″-O-p-hydroxybenzoyl)-β-D-glucoside( 1),2,3,5,4'-tetrahydroxystilbene-2-O-β-Dglucoside( 2),2,4,6,4'-tetrahydroxystilbene-2-O-β-D-glucoside( 3),emodin-8-O-β-D-glucopyranoside( 4),physcion-8-O-β-D-glucopyranoside( 5),and 8-O-methyl-emodin( 6) on the basis of spectral analysis. Compound 1 is a new stilbene glycoside.

关键词(KeyWords)： 何首乌;化学成分;2,3,5,4'-四羟基二苯乙烯-2-O-(2″-O-对羟基苯甲酰基)-β-D-葡萄糖苷
Polygonum multiflorum;chemical constituents;2,3,5,4'-tetrahydroxystilbene-2-O-(2″-O-p-hydroxybenzoyl)-β-D-glucoside

Abstract:

Keywords:

基金项目(Foundation): 国家“重大新药创制”科技重大专项(2012ZX09301002001003)

作者(Author): 张静娴;崔艳梅;
ZHANG Jing-xian;CUI Yan-mei;State Key Laboratory of Bioactive Substance and Function of Natural Medicines,Ministry of Education & Institute of Materia Medica,Chinese Academy of Medical Science and Peking Union Medical College;

Email:

DOI:

参考文献(References)：

• [1]中国药典.一部[S].2015:175.
• [2]张志国,吕泰省,姚庆强,等.何首乌蒽醌类化学成分研究[J].中草药,2006,37(9):1311.
• [3]Leung Y T,Lu K S Y,Zhang G H,et al.Identification and determination of themajor constituents in traditional Chinese medicinal plant Polygonum multiflorum Thumb.by HPLC coupled with PAD and ESI/MS[J].Phytochem Anal,2007(18):181.
• [4]苗明三,方晓艳.制何首乌多糖对衰老模型小鼠抗氧化作用的研究[J].中药药理与临床,2002,18(5):23.
• [5]高暄,胡英杰,符林春.何首乌二苯乙烯苷的调节血脂作用[J].中国中药杂志,2007,32(4):323.
• [6]Lv L S,Gu X H,Tang J,et al.Antioxidant activity of stilbene glycoside from Polygonum multiflorum Thunb in vivo[J].Food Chem,2007,104:1678.
• [7]Chen W S,Zhang W D,Yang G J,et al.Two new gucosides from Radix Polygoni multiflori preparata[J].Chin Chem Lett,2001,12(6):503.
• [8]Yang X W,Gu Z M,Ma C M,et al.A new indole derivative isolated from the root of Tuber Fleece-flower(Polygonum multiflorum)[J].Chin Tradit Herb Drugs,1998,29(1):5
• [9]Grech J N,Li Q,Roufogalis B D,et al.Novel Ca2+-ATPase inhibitors from the dried root tubers of Polygonum multiflorum[J].J Nat Prod,1994,57(12):1682.