中国中药杂志

2013, v.38(16) 2682-2689

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指纹图谱结合一测多评模式在中药鱼腥草质量评价中的应用研究
Study on quality control of Houttuynia Cordata,a traditional Chinese medicine by fingerprint combined with quantitative analysis of multi-components by single marker

何兵;刘艳;田吉;李春红;杨世艳;
HE Bing1,LIU Yan1,TIAN Ji1,LI Chun-hong1,YANG Shi-yan2*(1.Research Center for Drug,Luzhou Medical College,Luzhou 646000,China; 2.Hospital(T.C.M.)Affiliated to Luzhou Medical College,Luzhou 646000,China)

摘要(Abstract):

研究中药质量评价中指纹图谱与一测多评结合的考察模式,以中药鱼腥草为研究对象,以绿原酸为参照物建立鱼腥草HPLC指纹图谱及其与其余成分的相对校正因子,并计算其含量,实现一测多评。同时采用外标法测定该7个成分的含量,并比较二者的差异,以验证一测多评法的准确性和可行性。结果建立了鱼腥草HPLC特征指纹图谱共有模式,标定了12个共有峰,指认了其中7个共有峰,20批鱼腥草的相似度在0.912~0.991。20批鱼腥草中7个成分的计算值与实测值间无显著差异。结果表明一测多评结合指纹图谱的质量控制模式在鱼腥草中得到验证,有望成为适合中药特点的质量评价模式。
To establish a new method of quality evaluation of Traditional Chinese medicine by fingerprint and quantitative analysis of multi-components by single marker method(QAMS).The quality evaluation method was established and validated with Houttuyniae Herba.Chlorogcnic acid was selected as markers of ingredients to establish HPLC fingerprint and internal reference standard to determine the contents of other 6 components(new chlorogcnic acid,cryptochlorogenic acid,rutin,hyperin,isoquercitrin,quercitrin) according to the relative correction factor.At the same time,the seven components were determined by external standard method.The accuracy and feasibility of QAMS was evaluated by comparison of the results between external standard method and QAMS.All tested samples contained the 12 common peaks,7 of which was verified,and there was no significant differences between the quantitative results of 7 ingredients of multi-components by single marker method and external standard method in 20 batches.The method of fingerprint combined with QAMS has been verified in Houttuyniae Herba and it is to be a new quality evaluation pattern for Traditional Chinese medicine.

关键词(KeyWords): 指纹图谱;一测多评;质量控制;鱼腥草;高效液相色谱
fingerprint;quantitative analysis of multi-components by single marker(QAMS);quality control;Houttuyniae Herba;HPLC

Abstract:

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基金项目(Foundation): 泸州市2010年重点科技项目(泸市财企[2010]41号);; 泸州医学院2010年青年基金项目(泸医院[2010]108号)

作者(Author): 何兵;刘艳;田吉;李春红;杨世艳;
HE Bing1,LIU Yan1,TIAN Ji1,LI Chun-hong1,YANG Shi-yan2*(1.Research Center for Drug,Luzhou Medical College,Luzhou 646000,China; 2.Hospital(T.C.M.)Affiliated to Luzhou Medical College,Luzhou 646000,China)

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