张丹;郑开颜;吴兰芳;赵润怀;侯芳洁;王乾;由会玲;杜丽红;黄璐琦;郑玉光;
ZHANG Dan;ZHENG Kai-yan;WU Lan-fang;ZHAO Run-huai;HOU Fang-jie;WANG Qian;YOU Hui-ling;DU Li-hong;HUANG Lu-qi;ZHENG Yu-guang;College of Pharmacy,Hebei University of Chinese Medicine;China National Group Corp.of Traditional & Herbal Medicine;State Key Laboratory of Dao-di Herbs,National Resources Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;

• 1:河北中医学院药学院
• 2:中国药材公司
• 3:中国中医科学院中药资源中心道地药材国家重点实验室培育基地

摘要(Abstract)：

利用高效液相色谱法(HPLC)结合系统聚类分析(HCA)和主成分分析(PCA)等现代化学计量法,分析了西洋参药材传统商品规格特征与7种人参皂苷类成分(人参皂苷Rg_1,Re,Rb_1,Rc,Rb_2,Rb_3,Rd)定量指标之间的关联,探索西洋参传统商品规格特征与现代化学定量指标之间的关联性,为综合评价西洋参药材的质量等级的制定提供理论基础。采用HPLC指纹图谱法对40批西洋参药材进行分析,共标定19个峰,所有样品的相似度均在0.900以上。在此基础上,以加工方法、商品规格、7种成分质量分数、人参皂苷Rg_1,Re,Rb_1质量分数之和作为原变量进行聚类分析及主成分分析,结果表明所收集的西洋参药材质量与产地信息相关性较大,而与传统商品规格特征差别较小,说明西洋参药材传统商品规格的划分具有一定的局限性。研究结果揭示了西洋参药材商品规格与传统定性指标、与化学成分定量指标之间的内在关系,对西洋参药材质量进行客观综合定量评价提供了新思路。
[Abastract] This study was aimed to analyze the correlation between commercial specifications of Panacis Quinquefolii Radix and quantitative indexes of sevent kinds of ginsenosides( ginsenosides Rg_1,Re,Rb_1,Rc,Rb_2,Rb_3,Rd) contained in Panacis Quinquefolii Radix by using high performance liquid chromatography( HPLC),explore the correlation between the characteristics of the traditional Panacis Quinquefolii Radix specifications and modern chemical quantitative indicators,and provide a theoretical basis for the quality grade evaluation of Panacis Quinquefolii Radix. The HPLC fingerprint method was used to analyze 40 batches of Panacis Quinquefolii Radix. A total of 19 peaks were marked,and the similarity was above 0. 900 for all samples. On this basis,processing methods,product specifications,contents of 7 components,and the total contents of ginsenoside Rg_1,Re and Rb_1 were used as the original variables for cluster analysis and principal component analysis. The results showed great correlation between the quality of Panacis Quinquefolii Radix and the information on their origins,but the difference was less with the characteristics of traditional commercial specifications,indicating some limitations in the division of commercial specifications of Panacis Quinquefolii Radix. The results re-vealed the intrinsic relationship between the product specifications,traditional qualitative indexes,and quantitative indexes of chemical components of Panacis Quinquefolii Radix,providing a new idea for the objective comprehensive evaluation of the quality of Panacis Quinquefolii Radix.

关键词(KeyWords)： 西洋参;HPLC;指纹图谱;商品规格;质量评价
Panacis Quinquefolii Radix;HPLC;fingerprint;grade specification;quality evaluation

Abstract:

Keywords:

基金项目(Foundation): 中央本级重大增减支项目(2060302);; 河北省中医药管理局科研计划项目(2015079);; 河北中医学院博士科研基金项目(BSZ2015004)

作者(Author): 张丹;郑开颜;吴兰芳;赵润怀;侯芳洁;王乾;由会玲;杜丽红;黄璐琦;郑玉光;
ZHANG Dan;ZHENG Kai-yan;WU Lan-fang;ZHAO Run-huai;HOU Fang-jie;WANG Qian;YOU Hui-ling;DU Li-hong;HUANG Lu-qi;ZHENG Yu-guang;College of Pharmacy,Hebei University of Chinese Medicine;China National Group Corp.of Traditional & Herbal Medicine;State Key Laboratory of Dao-di Herbs,National Resources Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;

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DOI:

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