中国中药杂志

2020, v.45(19) 4555-4560

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原生质体及其在中药品质形成分子机制中的应用
Protoplast and its application in molecular mechanism of quality formation of traditional Chinese medicine

陈江;王洁;吴清华;马云桐;裴瑾;
CHEN Jiang;WANG Jie;WU Qing-hua;MA Yun-tong;PEI Jin;College Pharmacy, Chengdu University of Traditional Chinese Medicine;

摘要(Abstract):

原生质体自首次分离以来,在植物生物学及分子生物学等基础和应用研究中发挥了重要作用。原生质体的制备和转化,特别是在外植体的选择、酶解条件和转化方式上,有大量研究报道。原生质体在基因表达和调控,尤其是蛋白亚细胞定位、蛋白互作及启动子活性等研究中,也有广泛的应用。该文综述了原生质体制备和转化以及其在基因表达和调控中相关研究报道,概述了原生质体在中药品质形成分子机制中的研究现状,同时展望了原生质体在中药品质形成分子机制研究中重点方向:进一步加强对不同中药资源原生质体制备和转化条件摸索;进一步加强利用原生质体对品质形成相关功能基因的研究;进一步加强利用原生质体对中药品质形成分子调控的研究。该文试图为今后中药品质形成分子机制的解析提供新的研究思路和技术方法。
Since the first separation, protoplasts have played an important role in basic and applied research of plant biology and molecular biology. There are a lot of reports on protoplast preparation and transformation, especially in the selection of explants, enzymatic environment and transformation mode. Protoplasts have also been widely used in gene expression and regulation, especially in subcellular localization, protein interaction and promoter activity. In this paper, the preparation and transformation of protoplasts and the related research reports in gene expression and regulation are reviewed. The research status of protoplast in the molecular mechanism analysis of quality formation of traditional Chinese medicine is summarized. At the same time, the researches of protoplast in the molecular mechanism research of quality formation of traditional Chinese medicine are prospected. Firstly, we should further strengthen the exploration of protoplast preparation and transformation conditions of different traditional Chinese medicine resources. Secondly, we should further strengthen the research on quality formation related functional genes by using protoplast. Third, we should further make the study on the molecular regulation of quality formation of traditional Chinese medicine by protoplast. This paper attempts to provide a new way of thinking and technical method for the analysis of molecular mechanism of traditional Chinese medicine quality formation in the future.

关键词(KeyWords): 原生质体;中药;品质形成;分子机制
protoplast;traditional Chinese medicine;quality formation;molecular mechanism

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金面上项目(81573544);国家自然科学基金青年基金项目(81803669);; 中国博士后科学基金项目(2018M643790XB);; 四川省教育厅高校科研究创新团队——中药鉴定新技术创新团队项目(18TD0017)

作者(Author): 陈江;王洁;吴清华;马云桐;裴瑾;
CHEN Jiang;WANG Jie;WU Qing-hua;MA Yun-tong;PEI Jin;College Pharmacy, Chengdu University of Traditional Chinese Medicine;

Email:

DOI: 10.19540/j.cnki.cjcmm.20200623.101

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