中国中药杂志

2019, v.44(15) 3349-3357

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

云南白药配伍环境中草乌活血化瘀作用的血中移行成分研究
Study on absorbed components of Aconitum kusnezoffii under Yunnan Baiyao compatibility in effect of activating blood circulation and removing blood stasis

杨波;韩莹;张清宇;董辉;孙晖;王喜军;
YANG Bo;HAN Ying;ZHANG Qing-yu;DONG Hui;SUN Hui;WANG Xi-jun;National Chinmedomics Research Center,Heilongjiang University of Chinese Medicine;

摘要(Abstract):

采用皮下注射盐酸肾上腺素的方法复制血瘀证大鼠模型,应用云南白药治疗血瘀证大鼠,以血液流变学、凝血功能及组织病理学为指标评价血瘀证模型的成功复制及云南白药的治疗效果;采用超高效液相色谱串联高分辨飞行时间质谱技术(UPLC-Q-TOF-MS)快速分析云南白药治疗有效状态下大鼠血清中成分,结合Progenesis QI软件表征云南白药配伍环境中草乌的生物碱类成分。结果显示云南白药能降低血瘀证大鼠血浆黏度及全血黏度,延长凝血酶及凝血酶原时间,降低纤维蛋白原含量,同时能有效改善血瘀证大鼠各脏器瘀血、充血、水肿及炎细胞浸润等病理状态。在此状态下,鉴定了云南白药治疗血瘀证的7个草乌生物碱类入血成分,分别为多根乌头定碱、森布星B、异塔拉定、多根乌头碱、裸翠雀亭、塔拉乌头胺和展花乌头宁。结果表明在云南白药治疗血瘀证有效性的基础上,草乌表达了其治疗血瘀证的体内直接作用物质为多根乌头定碱、森布星B、异塔拉定、多根乌头碱、裸翠雀亭、塔拉乌头胺和展花乌头宁等生物碱类,为草乌在云南白药中的活血化瘀作用药效物质基础。
Rat model of blood stasis syndrome was prepared by subcutaneous injecting of epinephrine hydrochlorid,then the model rats were administrated by Yunnan Baiyao for 15 days. Blood rheology,coagulation function and histopathology were chosen as indicators to evaluate the successful replication of blood stasis syndrome model and the treatment effect of Yunnan Baiyao. UPLC-Q-TOF-MS was used to rapidly analyze the serum samples of blood stasis syndrome rat after 15 days Yunnan Baiyao treatment,Progenesis QI software was employed to identify the alkaloids components. The results showed that Yunnan Baiyao reduced the plasma viscosity and whole blood viscosity of rats with blood stasis syndrome,prolonged thrombin and prothrombin time,reduced fibrinogen content,and effectively improved pathological state such as inflammatory cell infiltration,blood stasis,congestion and edema of various organs in rats with blood stasis syndrome. Seven alkaloids components from Aconitum kusnezoffii,including karacolidine,senbusine B,isotalatizidine,karakoline,denudatine,talatisamine and chasmanine were found in the rat serum after Yunnan Baiyao treatment. Based on the effectiveness of Yunnan Baiyao in the treatment of blood stasis syndrome induced by epinephrine hydrochloride in rats,alkaloids components from the root of A. kusnezoffii absorbed into blood after Yunnan Baiyao treatment were clarified rapidly and accurately with the help of UPLC-Q-TOF-MS. Karacolidine,senbusine B,isotalatizidine,karakoline,denudatine,talatisamine and chasmanine are the pharmacodynamic material basis of the root of A. kusnezoffii for activating blood circulation and removing blood stasis.

关键词(KeyWords): 云南白药;草乌;血中移行成分;高效液相色谱串联高分辨质谱
Yunnan Baiyao;Aconitum kusnezoffii;constituents absorbed into blood;UPLC-Q-TOF-MS

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金重点项目(81430093);; 国家中医药管理局公益性行业科研专项(2015468004);; 黑龙江省自然科学基金青年基金项目(QC2018117);; 黑龙江省留学回国人员择优资助项目(2017QD0025);; 黑龙江中医药大学优秀创新人才支持计划项目(2018RCQ13)

作者(Author): 杨波;韩莹;张清宇;董辉;孙晖;王喜军;
YANG Bo;HAN Ying;ZHANG Qing-yu;DONG Hui;SUN Hui;WANG Xi-jun;National Chinmedomics Research Center,Heilongjiang University of Chinese Medicine;

Email:

DOI:

参考文献(References):

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享