中国中药杂志

2018, v.43(06) 1188-1191

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蛴螬的TLC鉴别与HPLC含量控制方法
Study on quality control of Holotrichia diomphalia larvae by TLC and HPLC

孙艳涛;孙静;王路宏;赵磊;
SUN Yan-tao;SUN Jing;WANG Lu-hong;ZHAO Lei;College of Chemistry,Jilin Normal University;Siping Institute for food and Drug Control;

摘要(Abstract):

建立蛴螬的TLC鉴别与蛴螬中肌苷、鸟苷含量同时测定的HPLC方法。采用薄层色谱分析用硅胶G板对蛴螬进行定性鉴别;采用色谱条件:色谱柱为Waters HSS T3(4.6 mm×250 mm,5μm);流动相为乙腈(A)和0.08%三氟乙酸(B)进行梯度洗脱;检测波长为260 nm;流速为1.0 m L·min~(-1);柱温为30℃。TLC鉴别方法重现性好,专属性强。含量测定中的肌苷和鸟苷的线性关系良好(r>0.999 8);平均回收率分别为96.53%(RSD 1.6%)和99.71%(RSD 2.7%)。建立方法简单、准确、重现性好,可为该药材的质量标准提高提供参考。
The TLC method was established for identification of Holotricha diomphalia larvae and the HPLC method was used to determine the content of inosine and guanosine in H. diomphalia larvae. The HPLC analysis was performed on a Waters HSS T3( 4. 6 mm × 250 mm,5 μm) column of with mobile phase consisting of acetonitrile( A) and 0. 08% trifluoroacetic acid( B) in gradient elution. The detection wavelength was 260 nm. The flow rate was 1. 0 m L·min~(-1). The column temperature was 30 ℃. As a result,TLC identification method had a good reproducibility and highly specificity. The linear equations of inosine and guanosine were in good linear range( r > 0. 999 8). The average recovery of inosine and guanosine was 96. 53%( RSD = 1. 6%),99. 71%( RSD = 2. 7%).The method is simple,accurate and reproducible,which can provide a basis for quality standard improvement H. diomphalia larvae.

关键词(KeyWords): 蛴螬;薄层鉴别;高效液相色谱;肌苷;鸟苷;含量测定
Holotricha diomphalia larvae;TLC;HPLC;inosine;guanosine;determination

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(61405071);; 2017吉林省质监局中药材标准制定修订项目;; 2017吉林省药监局中药材标准制定修订项目

作者(Author): 孙艳涛;孙静;王路宏;赵磊;
SUN Yan-tao;SUN Jing;WANG Lu-hong;ZHAO Lei;College of Chemistry,Jilin Normal University;Siping Institute for food and Drug Control;

Email:

DOI: 10.19540/j.cnki.cjcmm.2018.0044

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