中国中药杂志

2007, (15) 1518-1521

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

黄芩炭炮制工艺及其HPLC指纹图谱研究
Processing method and HPLC fingerprint of Charred Radix Scutellariae

杨云;闻永举;王俊敏;田润涛;
YANG Yun1,WEN Yong-ju1,WANG Jun-min1,TIAN Run-tao2(1.Henan College of Traditional Chinese Medicine,Henan,Zhengzhou 450008,China;2.Chromap Institute of Herbal Medicine,Zhuhai 519085,China)

摘要(Abstract):

目的:对黄芩炭的炮制工艺及其指纹图谱进行研究,为黄芩炭质量控制模式的建立奠定基础。方法:正交试验及HPLC-UV指纹图谱。色谱条件:Hypersil C18柱(5.0 mm×200 mm,5μm),流动相甲醇-0.4%磷酸水溶液-乙腈线性梯度洗脱;流速1.0 mL.m in-1;检测波长277 nm。结果:不同产地正品黄芩通过规范炮制加工后得到的黄芩炭指纹图谱无明显差异。结论:通过正交试验确定的黄芩炭制工艺切实可行,指纹图谱测定方法重现性好,可为黄芩炭的规范化炮制及质量控制提供可靠的科学依据。
Objective: To investigate the process and HPLC fingerprint of Charred Radix Scutellariae,and lay a foundation of Charred Radix Scutellariae quanlity control mode.Method: Select HPLC-UV fingerprint.Chromatogram condition: Hypersil C18 column(5.0 mm×200 mm,5 μm),mixtures of methanol,0.4% phosphoric acid and acetonitrile as mobile phase in a gradient mode.Flow rate: 1.0 mL·min;Detection wavelength was set at 277 nm.Result: There were no evident differences among Charred Radix Scutellariae that were normatively manufactured and processed.Conclusion: This test proves the process is feasible,this method recurs well and can be used to provide scieitific basis for the normative process and quanlity control mode of Charred Radix Scutellariae.

关键词(KeyWords): 黄芩炭;正交试验;HPLC指纹图谱
Charred Radix Scutellariae;orthogonal test;HPLC fingerprint

Abstract:

Keywords:

基金项目(Foundation): 河南省重大科技攻关项目(200422030700)

作者(Author): 杨云;闻永举;王俊敏;田润涛;
YANG Yun1,WEN Yong-ju1,WANG Jun-min1,TIAN Run-tao2(1.Henan College of Traditional Chinese Medicine,Henan,Zhengzhou 450008,China;2.Chromap Institute of Herbal Medicine,Zhuhai 519085,China)

Email:

DOI:

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享