黄芪中不同提取成分对在体大鼠心肌缺血-再灌注损伤的心功能影响Effects of Components Isolated from Astragalus membranaceus Bunge on Cardiac Function Injured by Myocardial Ischemia Reperfusion in Rats
周吉燕,樊懿,孔建龙,吴大正,胡之璧
ZHOU Ji-yan1, FAN Yi2, KONG Jian-long2, WU Da-zheng2, HU Zhi-bi2 (1.Scientific Research Office, Shanghai University of TCM, Shanghai 200032,China; 2. Institute of Chinese Materia Medica, Shanghai University of TCM, Shanghai 200032, China)
摘要(Abstract):
目的 :观察黄芪中不同提取成分对心肌缺血 再灌注损伤大鼠心功能的影响。方法 :通过结扎大鼠左室冠状动脉前降支造成心肌缺血 再灌注损伤模型 ,观察静脉内注射黄芪中不同提取成分对心肌缺血 再灌注损伤过程中血流动力学和缺血心肌Na+ K+ ATP酶活性、cAMP和丙二醛含量的影响。结果 :黄芪甲甙、总皂甙和总黄酮能够减轻心肌缺血 再灌注引起的左心室内压、左心室内压最大上升与下降速率振幅的下降 ;并能使缺血心肌组织中Na+ ,K+ ATP酶活性降低。另外 ,黄芪总皂甙能够增加缺血心肌组织中cAMP含量 ;黄芪总黄酮能够降低缺血心肌组织中丙二醛的含量。结论 :黄芪中不同提取成分对大鼠心肌缺血 再灌注引起心功能损害具有保护作用 ,其机制可能涉及到能量代谢的改善或自由基生成的抑制和氧自由基的清除。
Objective: To investigate the effects of components isolated from Astragalus membranaceus on myocardial ischemia reperfusion injury. Methods: The myocardial ischemia reperfusion injury model was created by the left anterior descending coronary artery occlusion from the oracotouated rats, and the total saponins, total flavonids and astragaloside IV isolated from A. membranaceus on hemodynamics during acute myocardial ischemia ,Na+-K+-ATPase activity, cAMP and malondialdehyede (MDA) contents in the ischemic myocardium were observed Results: The total saponins, total flavonids and astragaloside IV attenuated the declines of the amplitudes of LVSP and ±LVdp/dt max in rat heart injured by ischemia reperfusion in vivo, and decreased Na+-K+-ATPase activity in the ischemic myocardium. Otherwise, the total saponins increased the cAMP content and the total flavonids decreased the level of MDA production in the ischemic myocardium. Conclusion: The effects of different components isolated from A. membranaceus on protecting the cardiac function in the process of ischemia reperfusion may be related to the mechanism of improving energy metabolism, scavenging the oxygen free radicals and inhibiting the production of free radicals in the ischemic myocardium.
关键词(KeyWords):
黄芪;缺血-再灌注;心功能
Astragalus membranaceus; ischemia reperfusion; cardiac function
基金项目(Foundation):
作者(Author):
周吉燕,樊懿,孔建龙,吴大正,胡之璧
ZHOU Ji-yan1, FAN Yi2, KONG Jian-long2, WU Da-zheng2, HU Zhi-bi2 (1.Scientific Research Office, Shanghai University of TCM, Shanghai 200032,China; 2. Institute of Chinese Materia Medica, Shanghai University of TCM, Shanghai 200032, China)
Email:
DOI:
参考文献(References):
- [1] 何熹延 ,何吟锦 ,方蕴春 黄芪治疗心脑血管病的现代研究 南京 :南京大学出版社 ,1998.7.
- [2 ] 雷春利 ,陈 羽 ,吕文伟 ,等 黄芪总皂甙对犬急性心肌梗塞缺血面积、心外膜电图、心肌酶的影响 白求恩医科大学学报 ,1995 ,2 1( 2 ) :111.
- [3 ] 聂克 黄芪当归药对对心血管系统作用的研究概况 中国中西医结合杂志 ,1996,16( 6) :3 79.
- [4] HongCY ,LoYC ,TanFC ,etal.AstragalusMembranaceusandPolygonumMultiflorumProtectRatHeartMitochondriaA gainstLipidPeroxidation .AmJChinMed ,1994,12 ( 1) :63 .
- [5 ] 雷立权 ,韩启德 ,高文道 ,等 大鼠左室心肌收缩性能指标的测定 生理学报 ,1982 ,3 4 ( 3 ) :3 60 .
- [6] 金正均 ,王永铭 ,苏定冯 药理学进展 北京 :人民卫生出版社 ,1997.5 9.
- [7] 雷正一 ,王硕仁 黄芪心血管系统的作用 中国中西医结合杂志 ,1993 ,13 ( 7) :443 .
- [8] 冯国清 ,秦晓春 ,刘 洁 ,等 黄芪对大鼠心肌缺血 再灌注损伤的防护作用 中药药理与临床 ,1997,13 ( 3 ) :2 7.
- [9] 陈立新 ,廖家桢 ,郭维琴 ,等 黄芪对心肌梗塞患者心功能和氧自由基的影响及黄芪强心机制探讨 中国中西医结合杂志 ,1995 ,15 ( 3 ) :14 1.
- 周吉燕
- 樊懿
- 孔建龙
- 吴大正
- 胡之璧
ZHOU Ji-yan1 - FAN Yi2
- KONG Jian-long2
- WU Da-zheng2
- HU Zhi-bi2 (1.Scientific Research Office
- Shanghai University of TCM
- Shanghai 200032
- China
- 2. Institute of Chinese Materia Medica
- Shanghai University of TCM
- Shanghai 200032
- China)
- 周吉燕
- 樊懿
- 孔建龙
- 吴大正
- 胡之璧
ZHOU Ji-yan1 - FAN Yi2
- KONG Jian-long2
- WU Da-zheng2
- HU Zhi-bi2 (1.Scientific Research Office
- Shanghai University of TCM
- Shanghai 200032
- China
- 2. Institute of Chinese Materia Medica
- Shanghai University of TCM
- Shanghai 200032
- China)