中国中药杂志

2020, v.45(14) 3489-3496

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中药多糖质量评控方法探析
Analysis on quality evaluation and control methods of Chinese medicine polysaccharide

张文晋;王升;黄璐琦;郭兰萍;
ZHANG Wen-jin;WANG Sheng;HUANG Lu-qi;GUO Lan-ping;Joint Laboratory of Infinitus (China) Herbs Quality Research, State Key Laboratory of Dao-di Herbs Breeding Base,National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences;

摘要(Abstract):

中药多糖是重要的活性生物大分子,应用前景广阔。但目前多糖质量评控尚存在很大不足。该文结合中药多糖的质控现存问题,对中药多糖的提取、分离和纯化、特征鉴别、含量测定及结构分析方法进行了探析,得出以下结论:①基于中药在临床上以煎煮服药方式为主,中药多糖药效物质基础研究中,提取方法推荐煎煮法;②在除杂的基础上,HPGPC能同时实现中药多糖的分离纯化和含量测定,辅以MS或NMR法可实现中药多糖的定量定性分析;③以特征鉴别为依据,选择适宜的纯多糖、葡聚糖或单糖对照品;④HPSEC-MALLS-RID专属性、准确性高,且有利于多糖的构效机制研究。建议中药多糖质量综合评控应以提取分离纯化为基础,以特征鉴别为前提,以含量测定为重点,辅以结构分析,旨在为中药多糖质量研究提供有益借鉴。
Chinese medicine polysaccharide is an important active biological macromolecule, which has a broad application prospect. However, there are still many deficiencies in the quality evaluation and control of polysaccharides. Based on the existing problems in the quality control of traditional Chinese medicine polysaccharides, current review analyzes the methods of extraction, separation and purification, characteristic identification, content determination and structure analysis of Chinese medicine polysaccharides, and draws the following conclusions: ①Based on the clinical application of Chinese medicine, decoction is recommended as the extraction method in the basic study of effective substances of Chinese medicine polysaccharides; ②On the basis of impurity removal, HPGPC can achieve the separation, purification and content determination of Chinese medicine polysaccharides at the same time, supplemented by MS or NMR can achieve the quantitative and qualitative analysis of Chinese medicine polysaccharides; ③Based on the characteristic identification, select the suitable pure polysaccharide, dextran or monosaccharide reference; ④HPSEC-MALLS-RID is specific, accurate, and beneficial to the study of structure-activity mechanism of polysaccharides. The review suggested that the comprehensive evaluation and control of the quality of Chinese medicine polysaccharides should be based on extraction, separation and purification, on the premise of characteristic identification, on the basis of content determination, supplemented by structural analysis, which is to provide useful reference for the quality research of Chinese medicine polysaccharides.

关键词(KeyWords): 中药多糖;提取纯化;检测方法;质量评控
polysaccharide of Chinese medicine;extraction and purification;detection methods;quality evaluation and control

Abstract:

Keywords:

基金项目(Foundation): 国家重点研发计划项目(2017YFC1700701);; 国家自然科学基金重大项目(81891014);; 中国中医科学院重点领域研究项目(ZZ10-027);; 中央本级重大增减支项目(2060302);; 现代农业产业技术体系建设专项(CARS-21)

作者(Author): 张文晋;王升;黄璐琦;郭兰萍;
ZHANG Wen-jin;WANG Sheng;HUANG Lu-qi;GUO Lan-ping;Joint Laboratory of Infinitus (China) Herbs Quality Research, State Key Laboratory of Dao-di Herbs Breeding Base,National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences;

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