中国中药杂志

2004, (07)

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高效液相色谱法测定乌药中乌药醚内酯的含量
Determination of linderane in root tuber of Lindera aggregata by HPLC

蔡立红,侴桂新,王峥涛,李永国,徐德然,徐珞珊
CAI Li-hong1 ,CHOU Gui-xin1, WANG Zheng-tao1, LI Yong-guo1, XU De-ran2, XU Luo-shan2 (1.Shanghai Reaserch Centre for Standardization of Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; 2.Department of Parmacognosy, China Pharmaceutical University, Nanjing 210038, China)

摘要(Abstract):

目的 :建立乌药的质量标准 ,为乌药质量标准化提供研究的依据。方法 :采用反相高效液相色谱法 ,C18柱 ,流动相为乙腈 水 (5 6∶4 4 ) ,检测波长 2 35nm。结果 :乌药醚内酯线性范围为 0 .0 6 4 2~ 0 .5 774 μg ,r=0 .9999,平均回收率 98.4 %,RSD 1.7%(n =9)。结论 :以乌药醚内酯为定量指标 ,市售商品中乌药醚内酯含量为 0 .0 2 8%~0 .12 3%,自采样品中乌药醚内酯含量为 0 .0 5 6 %~ 0 .2 2 2 %。
Objective: To provide scientific basis for quality control of Lindera aggregata. Method: HPLC analytical method was established using a Lichrospher C 18 column and acetonitrile-water(56∶44) as the mobile phase,detected at 235nm. Result: The linear range of linderane is between 0.0642~0.5774 μg, the average recovery was 98.4%, RSD1.7%(n=9). Conclusion: Contents of linderane in commercially available and collected samples were from 0.028 % to 0.123% and from 0.056% to 0.222% respectivelly.

关键词(KeyWords): 乌药;乌药醚内酯;高效液相色谱;含量测定
Lindera aggreata; linderane; HPLC; quantitative detemination

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基金项目(Foundation): 上海市科学技术发展基金项目 ( 0 1DJ190 0 9);; 上海市重点学科建设项目资助

作者(Author): 蔡立红,侴桂新,王峥涛,李永国,徐德然,徐珞珊
CAI Li-hong1 ,CHOU Gui-xin1, WANG Zheng-tao1, LI Yong-guo1, XU De-ran2, XU Luo-shan2 (1.Shanghai Reaserch Centre for Standardization of Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; 2.Department of Parmacognosy, China Pharmaceutical University, Nanjing 210038, China)

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