中国中药杂志

2011, v.36(18) 2525-2529

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鸡血藤抗肿瘤活性部位SSCE指纹图谱的研究
Study on HPLC chromatographic fingerprint of anti-tumor active site SSCE of Caulis spatholobi

王宏;刘艺娜;曾祖平;何薇;
WANG Hong1,LIU Yina2,ZENG Zuping1,HE Wei1*(1.Beijing Institute of Traditional Chinese Medicine,Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University,Beijing 100010,China; 2.Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University,Beijing 100010,China)

摘要(Abstract):

目的:建立鸡血藤黄酮类抗肿瘤活性部位(SSCE)的HPLC-DAD色谱指纹图谱,全面完整的反映了本部位内在的化学信息,并初步指认其中的主要化学成分。方法:采用HPLC-DAD检测,梯度洗脱的分析方法。Kromasil 100-5PHENYL色谱柱(4.6 mm×250 mm,5μm);流动相0.5%冰醋酸水溶液-甲醇;检测波长254 nm。结果:建立了10批鸡血藤药材中SSCE的HPLC-DAD色谱指纹图,获得了16个共有峰,并有10个色谱峰被指认。其中,1,3,4,5,8,9,10,12,13,16号分别被确定为原儿茶酸,对羟基苯甲酸,表儿茶素,葛根素,大豆苷元,甘草素,毛蕊异黄酮,染料木素,芒柄花素和樱黄素。结论:方法简便,精密度、重现性和稳定性良好,能有效控制中药鸡血藤黄酮类抗肿瘤活性部位(SSCE)的质量,为筛选鸡血藤抗肿瘤活性成分、进行药理研究提供质量保证。
Objective: To establish the chromatographic fingerprints for the anti-tumor flavonoids of Caulis spatholobi(SSCE).It could used to reflect the chemical information in this part comprehensively,and identify the chemical consitituents preliminarily.Method: The HPLC-DAD analysis method was performed on the column Kromasil 100-5PHENYL(4.6 mm×250 mm,5 μm).The mobile phase was water(0.5% acetic acid)-methanol in gradient elution and the detection wavelength was 254 nm.Result: The chromatographic fingerprint of SSCE was established,which showed 16 characteristic peaks from 10 batches of medicinal materials.Among them,the peaks 1,3,4,5,8,9,10,12,13,and 16 were identified 3,4-dihodroxybenzoic acid,4-Hydroxybenzoic Acid,epicatechin,puerarin,daidzein,liquiritigenin,calycosin,genistein,formononetin,and prunetin,respectively.Conclusion: The method is convenient,reproducibility and stability.It can used for quality control of the anti-tumor flavonoids of C.spatholobi(SSCE).

关键词(KeyWords): HPLC;鸡血藤;中药指纹图谱;活性部位
HPLC;Caulis Spatholobi;Fingerprints;active site

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基金项目(Foundation): 北京市科技项目(H010910190119)

作者(Author): 王宏;刘艺娜;曾祖平;何薇;
WANG Hong1,LIU Yina2,ZENG Zuping1,HE Wei1*(1.Beijing Institute of Traditional Chinese Medicine,Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University,Beijing 100010,China; 2.Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University,Beijing 100010,China)

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