中国中药杂志

2019, v.44(24) 5352-5357

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4株蜜环菌菌株鉴定及其对彝良乌天麻产质量的影响
Identification of four Armillaria strains and their effects on quality and yield of Gastrodia elata f. glauca

刘天睿;王忠巧;陈向东;张薇薇;杨勇述;徐万雷;包海鹰;兰进;
LIU Tian-rui;WANG Zhong-qiao;CHEN Xiang-dong;ZHANG Wei-wei;YANG Yong-shu;XU Wan-lei;BAO Hai-ying;LAN Jin;Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences & Peking Union Medical College;Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi,Jilin Agricultural University;Tianma Industry Development Office of Yanliang;

摘要(Abstract):

为了保证乌天麻栽培品质提高乌天麻产量,筛选适合彝良小草坝乌天麻伴栽的蜜环菌菌株,该研究选择了4株不同的蜜环菌菌株,首先对其r DNA-ITS、β-tubulin和EF1-α基因片段扩增,并进行系统发育分析,同时采用"三下窝"的方式栽培乌天麻于当年11月采收。测定乌天麻产量并对乌天麻进行简单的蒸制加工,烘干后的粉末使用UPLC测定天麻素和对羟基苯甲醇的含量,苯酚-浓硫酸法测定天麻多糖的含量。结果表明4株蜜环菌菌株为高卢蜜环菌族Armillaria gallica,但伴栽的乌天麻产量和有效成分含量存在差异,在天麻产量方面,M3菌株伴栽的天麻箭麻产量最低,M1箭麻产量最高为0. 981 kg·m-2;在有效成分含量方面,M1菌株伴栽的天麻天麻素质量分数为0. 581%,天麻素和对羟基苯甲醇的总量0. 595%,二者均高于其他菌株,而天麻多糖质量分数为2. 132%,与M3菌株的含量相近,高于M2,M4。以乌天麻产量、天麻素、天麻素和对羟基苯甲醇的总含量及多糖含量作为评价指标,结合蜜环菌的系统发育筛选出彝良乌天麻伴栽的最适菌株为M1,研究结果为彝良乌天麻高产优质、规范化栽培提供了理论依据。
In order to improve the quality and yield of Gastrodia elata f. glauca,determine the suitable Armillaria strains for the accompanying experiment in Xiaocaoba,Yiliang,four Armillaria strains were selected. They were used for G. elata cultivation,and the gene sequence,r DNA-ITS,β-tubulin and EF1-α of four Armillaria strains,were compared and analyzed. The yield was mesured in November which was based on previous laboratory research. The tubers were washed and steamed,then dried and powdered. The content of gastrodin and p-hydroxybenzyl alcohol was determined by UPLC,the polysaccharide was determined by phenol-concentrated sulfuric acid method. The results showed that the strains M1,M2,M3 and M4 were Armillaria gallica group but there were differences in the yield and active ingredient content when they were cultivated with the same G. elata. The yield of G. elata( Jian Ma) was the lowest when cultivated with Armillaria strain M3,but it was not the same when used M1,0. 981 kg·m-2,the highest yield in the four stains.The content of gastrodin was 0. 581%,the total content of gastrodin and p-hydroxybenzyl alcohol was 0. 595%,when accompanied with M1 strains. It was higher than other strains. The content of G. elata polysaccharide was 2. 132%,which was similar to the content of M3 strain,higher than that of M2 and M4 strain. Selecting phylogenesis of Armillaria strians,the content of active ingredient,and the yield as indicators,it was concluded concerned that the M1 strain was the best of four strains. The results will provide a theoretical basis and guidance for higher yield and quality in cultivation of G. elata in Yiliang.

关键词(KeyWords): 乌天麻;蜜环菌;天麻产量;天麻素;天麻多糖
Gastrodia elata f.glauca;Armillaria sp.;the yield of Gastrodia elata;gastrodin;polysaccharide

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基金项目(Foundation): 国家自然科学基金项目(81573703)

作者(Author): 刘天睿;王忠巧;陈向东;张薇薇;杨勇述;徐万雷;包海鹰;兰进;
LIU Tian-rui;WANG Zhong-qiao;CHEN Xiang-dong;ZHANG Wei-wei;YANG Yong-shu;XU Wan-lei;BAO Hai-ying;LAN Jin;Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences & Peking Union Medical College;Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi,Jilin Agricultural University;Tianma Industry Development Office of Yanliang;

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