中国中药杂志

2019, v.44(15) 3178-3186

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基于转录组测序挖掘新塔花黄酮类物质生物合成相关基因
Transcriptome analysis reveals candidate genes involved in flavonoid biosynthesis in Ziziphora bungeana

何江;马艺沔;杨伟俊;程波;地力努尔;马丽娜;李冠;
HE Jiang;MA Yi-mian;YANG Wei-jun;CHENG Bo;DI Li-nu-er;MA Li-na;LI Guan;College of Life Science and Technology,Xinjiang University;Key Laboratory of Uighur Medicine,Xinjiang Institute of Materia Medica;Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College;

摘要(Abstract):

新塔花为唇形科植物,新疆地产药材,其主要药效成分为蒙花苷等黄酮类成分。该研究利用二代高通量测序技术对新塔花的花序部位进行转录组高通量测序,获得77 366条单一序列。通过搜索数据库和聚类分析对56 375条unigene进行了功能注释,继续对代谢通路分析,发现新塔花转录组中有60条unigene可能编码黄酮合成途径一些酶类。后对其不同组织的蒙花苷的含量及4条黄酮合成关键基因进行了测定、分析及验证,为进一步分析其他参与新塔花中黄酮类成分生物合成相关酶基因奠定了研究基础。
Ziziphora bungeana is a kind of medicinal plants belongs to Labiatae,and it also a kind of geoherbs in Xinjiang. The main active ingredient linarin has a higher content in inflorescence than in other parts. In this study,high-throughput sequencing technology was used to reveal the transcriptome of the inflorescence of Z. bungeana,77 366 unigenes were acquired,of which 56 375 unigenes were annotated based on search of the database and classification. Through the analysis of metabolic pathways,sixty unigenes were probably encoding some enzymes involved in the flavonoid biosynthesis pathways. The contents of linarin in different parts were determined and the key genes were verified by qRT-PCR. The discovery provides the research basis for further analysis of the enzyme genes involved in the biosynthesis of the major flavonoid components in Z. bungeana.

关键词(KeyWords): 新塔花;转录组;黄酮类;生物合成;单一序列
Ziziphora bungeana;transcriptome;flavonoids;biosynthesis;unigene

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基金项目(Foundation): 天山创新团队计划项目(2017D14014);; 国家自然科学基金地区基金项目(81860748)

作者(Author): 何江;马艺沔;杨伟俊;程波;地力努尔;马丽娜;李冠;
HE Jiang;MA Yi-mian;YANG Wei-jun;CHENG Bo;DI Li-nu-er;MA Li-na;LI Guan;College of Life Science and Technology,Xinjiang University;Key Laboratory of Uighur Medicine,Xinjiang Institute of Materia Medica;Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College;

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