中国中药杂志

2008, (14) 1717-1719

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延胡索抗心肌缺血活性部位指纹图谱中化学成分分析
Analysis of chemical constituents of anti-myocardial ischemia fraction of Corydalis yanhusuo

程星烨;石钺;
CHENG Xing-ye SHI Yue (Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Peking Union Medical College,Beijing 100094,China)

摘要(Abstract):

目的:运用LC-MS~2技术对延胡索抗心肌缺血活性部位指纹图谱中化学成分进行分析,初步了解其主要活性成分。方法:采用迪马公司Diamonsil C_(18)柱(4.6 mm×250 mm,5μm),流动相为乙腈-水(含0.8%冰醋酸和0.2%三乙胺23:77),流速1 mL·min~(-1),检测波长335nm,柱温30℃。结果:建立了延胡索抗心肌缺血活性部位的HPLC指纹图谱,确立了指纹图谱中的12个共有峰,根据所检测到化合物的色谱保留时间及多级质谱信息,与对照品比较,对其中8个色谱峰进行了指认。结论:本方法为延胡索抗心肌缺血活性部位的质量控制提供了依据。
Objective:To establish the HPLC fingerprint of anti-myocardial ischemia fraction of Corydalis yanhusuo,and to in- vestigate its chemical constituents by LC-MS-MS.Method:The separation was performed at 30℃on a Dikma Diamonsil C_(18)column (4.6 mm×250 mm,5μm)by isocratic elution with acetonitrile-water(23:77)( containing 0.8% acetic acid and 0.2% triethyl- amine)as the mobile phase at a flow rate of 1.0 mL·min~(-1),with UV detection at 335 rim.Result:A satisfactory fingerprint was ob- tained by HPLC method,12 common peaks were selected as the fingerprint peaks,and 8 among them were identified according to the chromatographic behavior and mass spectroscopic data by comparison with those of authentic standard.Conclusion:This method is simple,sensitive and reliable,can be used to investigate the chemical constituents of anti-myocardial ischemia fraction of C.yanhusuo and control the quality.

关键词(KeyWords): 延胡索;活性部位;LC-MS~2;心肌缺血;季铵碱
Corydalis yanhusuo;effective fraction;LC-MS-MS;myocardial ischemia;quaternary alkaloids

Abstract:

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基金项目(Foundation): 中央级公益型科研院所基本科研业务专项资助项目(YZ-1-28)

作者(Author): 程星烨;石钺;
CHENG Xing-ye SHI Yue (Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Peking Union Medical College,Beijing 100094,China)

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