王茜;侯飞侠;王艺璇;王玉贤;李军德;袁媛;彭成;国锦琳;
WANG Xi;HOU Fei-xia;WANG Yi-xuan;WANG Yu-xian;LI Jun-de;YUAN Yuan;PENG Cheng;GUO Jin-lin;Pharmacy College,Chengdu University of Traditional Chinese Medicine/The Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine / Key Laboratory of Systematic Research,Development and Utilization of Chinese Medicine Resources in Sichuan Province-Key Laboratory Breeding Base of Co-founded by Sichuan Province and Ministry of Science and Technology;State Key Laboratory of Dao-di Herbs

• 1:成都中医药大学中药材标准化教育部重点实验室四川省中药资源系统研究与开发利用重点实验室-省部共建国家重点实验室培育基地
• 2:中国中医科学院中药资源中心道地药材国家重点实验室培育基地

摘要(Abstract)：

市场调查与文献报道发现,螳螂目昆虫产的卵鞘大多作为桑螵蛸入药,而药典仅收载了3种螳螂的卵鞘,非药典品种的卵鞘入药由于未加评估可能影响临床用药安全,因此亟需研究其原昆虫,由于桑螵蛸是螳螂的卵鞘,形态学等方法无法直接判断其基原,基于DNA条形码技术在动物分类研究中广泛应用,在中药鉴定中也愈发重要。研究首次利用DNA条形码技术,获得了市售桑螵蛸的COI序列,分析了其遗传距离,并构建系统发育树,准确区分了桑螵蛸的种类和原昆虫,证实了大刀螂Tenodera sinensis和巨斧螳螂Hierodula patellifera分别为团螵蛸和黑螵蛸的基原昆虫,小刀螂Statilia maculate和薄翅螳螂Mantis religiosa均为长螵蛸的原昆虫。
Both market research and literature reports both found that the ootheca of mantodea was all used as medicine. However,Chinese Pharmacopoeia only records the ootheca of three mantis species. The clinical use of ootheca unrecorded in Chinese Pharmacopoeia,will pose potential risks to drug safety. It's urgent to identify the origin of Mantidis Otheca. The current researches about original animal in Mantidis Otheca are based on morphology and unanimous. DNA barcoding fill gaps of the traditional morphological identification,which is widely used in animal classification studies. This study first use DNA barcoding to analyze genetic distance among different Mantidis Otheca types,align COI sequences between mantis and Mantidis Otheca and construct the phylogeny tree. The result confirmed that Tenodera sinensis and Hierodula patellifera were the origin insects of Tuanpiaoxiao and Heipiaoxiao,respectively,and Statilia maculate and Mantis religiosa were the origin insects of Changpiaoxiao.

关键词(KeyWords)： 桑螵蛸;基原鉴别;螳螂;DNA条形码
Mantidis Otheca;origin identification;Mantis;DNA barcoding

Abstract:

Keywords:

基金项目(Foundation): 中医药行业专项(201407003);; 成都中医药大学科技发展基金项目(ZRQN1445)

作者(Author): 王茜;侯飞侠;王艺璇;王玉贤;李军德;袁媛;彭成;国锦琳;
WANG Xi;HOU Fei-xia;WANG Yi-xuan;WANG Yu-xian;LI Jun-de;YUAN Yuan;PENG Cheng;GUO Jin-lin;Pharmacy College,Chengdu University of Traditional Chinese Medicine/The Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine / Key Laboratory of Systematic Research,Development and Utilization of Chinese Medicine Resources in Sichuan Province-Key Laboratory Breeding Base of Co-founded by Sichuan Province and Ministry of Science and Technology;State Key Laboratory of Dao-di Herbs

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DOI:

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