中国中药杂志

2015, v.40(12) 2383-2388

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毛蕊异黄酮上调过氧化物酶体增殖因子活化受体γ抑制大鼠肝星状细胞活化的研究
Study on inhibitory effect of calycosin on hepatic stellate cell activation in rats by up-regulating peroxisome proliferator-activated receptor γ

平键;陈红云;周扬;陈高峰;徐列明;成扬;
PING Jian;CHEN Hong-yun;ZHOU Yang;CHEN Gao-feng;XU Lie-ming;CHENG Yang;Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine;Shanghai Key Clinical Laboratory of Traditional Chinese Medicine;Changhai Hospital of Traditional Chinese Medicine,Second Military Medical University;Hospital for Infectious Diseases,Pudong New District,Shanghai;

摘要(Abstract):

该研究观察了毛蕊异黄酮对大鼠原代肝星状细胞(HSC)增殖和活化的影响,并探讨其是否通过影响核因子过氧化物酶体增殖因子活化受体γ(PPARγ)和法尼酯衍生物X受体(FXR)发挥作用。结果发现毛蕊异黄酮能抑制HSC的增殖,抑制活化标志物α-平滑肌肌动蛋白和Ⅰ型胶原的表达;随着HSC活化时间的延长,FXR表达逐渐降低,但毛蕊异黄酮对FXR表达没有明显影响;毛蕊异黄酮可浓度依赖性地上调PPARγ的表达及核转位,PPARγ拮抗剂可阻断毛蕊异黄酮对HSC活化的抑制作用。因此,结论认为毛蕊异黄酮可抑制HSC增殖和活化,其作用不通过FXR而是与上调PPARγ信号通路有关。
To observe the effect of calycosin on the proliferation and activation of primary hepatic stellate cells(HSCs) in rats,and prove calycosin shows the effects through peroxisome proliferator-activated receptor γ( PPARγ) and farnesoid X receptor( FXR). The results indicated that calycosin could inhibit HSC proliferation and expressions of activation marker smooth muscle actin-α and type Ⅰcollagen. With the increase in HSC activation time,FXR expression reduced,but with no notable impact from calycosin. Calycosin could up-regulate PPARγ expression and its nuclear transition in a concentration-dependent manner. Its prohibitory effect on HSC activation could be blocked by PPARγ antagonist. In conclusion,calycosin could inhibit HSC activation and proliferation,which may be related with the up-regulation of PPARγ signal pathway.

关键词(KeyWords): 毛蕊异黄酮;肝星状细胞;过氧化物酶体增殖因子活化受体
calycosin;hepatic stellate cells(HSCs);peroxisome proliferator-activated receptor γ(PPARγ)

Abstract:

Keywords:

基金项目(Foundation): 上海市自然科学基金项目(10ZR1430700);; 上海市浦东新区科委项目(PKJ2014-Y37);; 上海市中医药三年行动计划项目(ZYSNXD)

作者(Author): 平键;陈红云;周扬;陈高峰;徐列明;成扬;
PING Jian;CHEN Hong-yun;ZHOU Yang;CHEN Gao-feng;XU Lie-ming;CHENG Yang;Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine;Shanghai Key Clinical Laboratory of Traditional Chinese Medicine;Changhai Hospital of Traditional Chinese Medicine,Second Military Medical University;Hospital for Infectious Diseases,Pudong New District,Shanghai;

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