中国中药杂志

2020, v.45(03) 463-471

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

天麻-冬荪轮种模式对土壤微生物群落结构的影响
Effect of Gastrodiae elata-Phallus impudicus sequential planting pattern on soil microbial community structure

徐娇;欧小宏;江维克;袁青松;王艳红;杨杰;刘大会;王晓;张光文;潘成;周涛;
XU Jiao;OU Xiao-hong;JIANG Wei-ke;YUAN Qing-song;WANG Yan-hong;YANG Jie;LIU Da-hui;WANG Xiao;ZHANG Guang-wen;PAN Cheng;ZHOU Tao;Guizhou University of Traditional Chinese Medicine;Hubei University of Chinese Medicine;Shandong Analysis and Test Center;Guizhou Wumeng Fungi Industry Co., Ltd.;

摘要(Abstract):

天麻-冬荪轮种模式是贵州产区采用的一种新型种植模式,但是天麻-冬荪的种植对土壤微生物群落结构的影响尚不清楚。因此,该研究采集未种植天麻土壤为对照(CK),分别采集天麻块茎际土壤(GE)、蜜环菌菌索区际土壤(AGE)、天麻-冬荪轮作后的白鬼笔Phallus impudicus菌丝区际土壤(PI),采用ITS和16S rDNA高通量测序技术对土壤真菌和细菌的群落组成进行测定分析。结果表明,通过OTU聚类和主成分(PCA)分析发现,AGE和GE土壤微生物聚为一类,PI和CK土壤微生物聚为一类;与CK相比,AGE和GE土壤微生物的多样性和丰度均显著增加;而与AGE和GE相比,PI土壤微生物的多样性和丰度均降低。物种注释表明,与CK相比,AGE和GE土壤中担子菌门Basidiomycota、酸杆菌门Acidobacteria、绿弯菌门Chloroflexi的丰度明显减少,而子囊菌门Ascomycota、接合菌门Zygomycota、变形菌门Proteobacteria丰度明显增加;与AGE和GE相比,PI则与之相反,PI与CK土壤微生物丰富度较为相似。此外,蜜环菌与冬荪菌共培养表明冬荪菌可以明显抑制蜜环菌的生长。由此推测,冬荪菌对土壤微生物群落结构的影响表现为通过抑制蜜环菌的生长,从而使土壤微生物群落结构得以恢复。
Gastrodia elata is a heterotrophic plant that needed to be symbiotic with Armillaria. The obstacle of continuous cropping in G. elata is serious during the G. elata cultivation, and the mechanism of obstacle in G. elata continuous cropping had not been solved. The planting of G. elata-Phallus impudicus is a new sequential planting pattern adopted in Guizhou province, but the effect of the cultivation on soil microbial community structure is still unclear. In this study, we collected four soil samples for the research including the soil without planted G. elata as control(CK), rhizosphere soil samples tightly adhering to the G. elata surface(GE), rhizosphere soil samples tightly adhering to Armillaria which was symbiotic with G. elata(AGE), the rhizosphere soil of P. impudicus planting after G. elata cultivation(PI). In order to explore the mechanism, the research study on the soil of G. elata-P. impudicus by using ITS and 16 S rDNA high-throughput sequencing technologies to detect soil microbial community structure including fungi and bacteria in the soil of CK, AGE, GE and PI. OTU clustering and PCA analysis of soil samples showed that the soil microbial diversity was relatively similar in AGE and GE. And the soil microbial in PI and CK clustered together. The results showed that AGE and GE had similar soil microbial diversity, as well as PI and CK. Compared with CK, the soil microbial diversity and abundance in AGE and GE were significantly increased. But the microbial diversity and abundance decreased in PI compared with AGE and GE. The annotation indicated that the abundance of Basidiomycota, Acidobacteria and Chloroflexi decreased, and that of Ascomycota, Zygomycota and Proteobacteria increased in AGE and GE compared with CK. In contrast to AGE and GE, PI was the opposite. The abundance of Basidiomycota, Acidobacteria and Chloroflexi increased in PI compared with AGE and GE. The abundance of microorganisms in the soil of PI and CK was similar. In addition, the co-culture of Armillaria and P. impudicus indicated that P. impudicus had obvious antagonistic effects on the growth of Armillaria. Therefore, it is speculated that the mechanism of G. elata-P. impudicus planting pattern related to the change of soil microbial. And we supposed that P. impudicus might inhibit the growth of Armillaria and change the soil microbial community structure and the abundance of soil microbial. And the soil microbial community structure was restored to a state close to that of uncultivated G. elata. Thus, the structure of soil microbial community planting G. elata could be restored by P. impudicus planting.

关键词(KeyWords): 天麻;冬荪;蜜环菌;土壤微生物
Gastrodiae elata;Phallus impudicus;Armillaria;soil microbial

Abstract:

Keywords:

基金项目(Foundation): 中央本级重大增减支项目(2060302);; 国家自然科学基金项目(81960694);; 国家重点研发计划中医药现代化研究专项(2017YFC1700703);; 贵州省科技创新人才团队项目(黔科合平台人才[2019]5611);; 贵州省高层次创新型人才项目(黔科合平台人才[2018]5638);; 贵州省教育厅创新群体重大研究项目(黔教合KY字[2018]022);; 国家中药材产业技术体系项目(CARS-21);; 贵州中医药大学博士启动基金项目([2019]32)

作者(Author): 徐娇;欧小宏;江维克;袁青松;王艳红;杨杰;刘大会;王晓;张光文;潘成;周涛;
XU Jiao;OU Xiao-hong;JIANG Wei-ke;YUAN Qing-song;WANG Yan-hong;YANG Jie;LIU Da-hui;WANG Xiao;ZHANG Guang-wen;PAN Cheng;ZHOU Tao;Guizhou University of Traditional Chinese Medicine;Hubei University of Chinese Medicine;Shandong Analysis and Test Center;Guizhou Wumeng Fungi Industry Co., Ltd.;

Email:

DOI: 10.19540/j.cnki.cjcmm.20191204.106

参考文献(References):

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享