杜俊潮;浦香兰;范恺磊;翟燕娟;张宇静;王协和;陈盛君;李松;张云天;陈可冀;彭军;许运明;
DU Jun-chao;PU Xiang-lan;FAN Kai-lei;ZHAI Yan-juan;ZHANG Yu-jing;WANG Xie-he;CHEN Sheng-jun;LI Song;ZHANG Yun-tian;CHEN Ke-ji;PENG Jun;XU Yun-ming;Jiangyin Tianjiang Pharmaceutical Co., Ltd.;Key Technology Laboratory for Preparation and Quality Control of Traditional Chinese Medicine Granules in Jiangsu;Jiangsu Traditional Chinese Medicine Formula Granule Engineering Technology Center;Jiangsu Traditional Chinese Medicine Formula Granule Engineering Research Center;Academy of Integrative Medicine

• 1:江阴天江药业有限公司
• 2:江苏省中药配方颗粒制备与质量控制关键技术重点实验室
• 3:江苏省中药配方颗粒工程技术中心
• 4:江苏省中药配方颗粒工程研究中心

摘要(Abstract)：

建立天麻标准汤剂中存在转化关系的6种成分的一测多评方法和指纹图谱,验证该方法的准确度及可行性,并对标准汤剂质量进行评价。采用超高效液相色谱技术,以天麻素为内标物,通过考察柱温、流速、不同色谱柱以及多点浓度校正,确定对羟基苯甲醇、巴利森苷E、巴利森苷B、巴利森苷C和巴利森苷与天麻素的相对校正因子。测定18批天麻标准汤剂总含量,计算相似度。比较标准曲线法,外标一点法和一测多评法测量结果,结果表明一测多评法,外标一点法和标准曲线法的含量测定结果无显著差异,通过分析不同产地标准汤剂结果可以看出,来源于安徽、云南制得天麻标准汤剂质量较优,陕西、湖北次之,甘肃相对较差,且多批次指纹图谱相似度均在0.90以上。以天麻素为内标物,一测多评测定天麻标准汤剂中天麻素、对羟基苯甲醇、巴利森苷E、巴利森苷B、巴利森苷C和巴利森苷等6个成分总含量,结合指纹图谱的方法准确、可行、快捷,可用于天麻标准汤剂质量评价体系的建立,亦可对天麻药材饮片等原料质量标准研究提供参考。
To establish the quantitative analysis multi-components with a single-marker(QAMS) method for six components and fingerprint of standard decoction of Gastrodiae Rhizoma, verify the accuracy and feasibility of the method, and evaluate the quality of standard decoction. Based on UPLC with gastrodin as the internal standard, relative correction factors of p-hydroxybenzyl alcohol, parishin E, parishin B, parishin C, parishin A and gastrodin were determined by investigating the column temperature, flow rate, chromatographic columns and multi-point concentration correction. The total contents in 18 batches of standard decoction of Gastrodiae Rhizoma and the similarity were determined to calculate the similarity. The results of standard curve method, external standard one-point method and quantitative analysis multi-components with a single-marker(QAMS) were compared, and the results showed that there was no significant difference among these three methods. By analyzing the results of standard decoctions from different origins, it can be seen that the quality of Gastrodia standard decoctions derived from Anhui and Yunnan was better, followed by Shaanxi and Hubei, and relatively poor in Gansu, with similarities all above 0.90 in the fingerprints. Therefore, the QAMS method that can measure the contents of gastrodin, p-hydroxybenzyl alcohol, parishin E, parishin B, parishin C and parishin A in standard decoction of Gastrodiae Rhizoma combined with fingerprint is accurate, feasible and fast, which can be used to evaluate the quality of standard decoction of Gastrodiae Rhizoma, and also provide a reference for the research on the quality standards of raw materials for Gastrodiae Rhizoma prepared slices and alike.

关键词(KeyWords)： 天麻;标准汤剂;一测多评;巴利森苷;指纹图谱
Gastrodiae Rhizoma;standard decoction;QAMS;parishin;fingerprints

Abstract:

Keywords:

基金项目(Foundation): 国家“重大新药创制”科技重大专项(2018ZX09721005-002-005,2018ZX09201011-004-004)

作者(Author): 杜俊潮;浦香兰;范恺磊;翟燕娟;张宇静;王协和;陈盛君;李松;张云天;陈可冀;彭军;许运明;
DU Jun-chao;PU Xiang-lan;FAN Kai-lei;ZHAI Yan-juan;ZHANG Yu-jing;WANG Xie-he;CHEN Sheng-jun;LI Song;ZHANG Yun-tian;CHEN Ke-ji;PENG Jun;XU Yun-ming;Jiangyin Tianjiang Pharmaceutical Co., Ltd.;Key Technology Laboratory for Preparation and Quality Control of Traditional Chinese Medicine Granules in Jiangsu;Jiangsu Traditional Chinese Medicine Formula Granule Engineering Technology Center;Jiangsu Traditional Chinese Medicine Formula Granule Engineering Research Center;Academy of Integrative Medicine

Email:

DOI: 10.19540/j.cnki.cjcmm.20200622.303

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