高燕;李文婕;李聪颖;范刚;张艺;
GAO Yan;LI Wen-jie;LI Cong-ying;FANG Gang;ZHANG Yi;College of Ethnic Medicine,Chengdu University of Traditional Chinese Medicine;

• 1:成都中医药大学民族医药学院

摘要(Abstract)：

建立不同产地藏药翼首草的UFLC指纹图谱分析方法。实验采用UFLC-PDA检测法,使用Agilent Proshell 120SB-C18色谱柱(4.6 mm×100 mm,2.7μm),以乙腈-0.2%磷酸水溶液为流动相梯度洗脱,流速1.0 mL·min-1,柱温为30℃,检测波长238 nm。建立了21个批次翼首草药材的共有图谱,提取了15个色谱峰作为指纹图谱共有峰,结合对照品对其中的5个色谱峰进行了指认,分别为绿原酸、马钱苷、獐芽菜苷、吴茱萸苷、大花双参苷A;21批药材中第13,18,20批次与对照指纹图谱比较相似度小于0.9,其他批次的相似度均大于0.9。该研究首次采用UFLC-PDA建立藏药翼首草的指纹图谱,该方法具有良好的精密度、稳定性和重复性,为翼首草质量控制和评价提供依据。
This study was aimed to establish an UFLC fingerprint of Tibetan medicine Pterocephalus hookeir samples from different habitats. UFLC-PDA was adopted to analyse 21 batches of P. hookeir samples from different habitats. The chromatographic condition was as follow: Agilent proshell 120 SB-C18column( 4. 6 mm × 100 mm,2. 7 μm) eluted with the mobile phases of acetonitrile and 0. 2% phosphoric acid water in gradient mode. The flow rate was 1. 0 mL·min-1,and the detection wavelength was set at 238 nm. The fingerprints of 21 batches P. hookeir were carried out by similarity comparation,and 15 chromatographic peaks were extracted as the common peaks of fingerprint,of which 5 peaks were identified as chlorogenic acid,loganin,sweroside,sylvestroside Ⅲ,triplostoside A. The similarity degrees of 18 batchs of samples were above 0. 9,and the other 3 batchs of samples were below 0. 9. This is the first established fingerprint of P. hookeir by using UFLC-PDA. This method has good precision,stability and repeatability that it could provide basis for quality control and evaluation of P. hookeir.

关键词(KeyWords)： 藏药;翼首草;UFLC-PDA;指纹图谱
Tibetan medicinal material;Pterocephalus hookeir;UFLC-PDA;chromatographic fingerprint

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81274193);; 四川省教育厅四川省省属高校科研创新团队建设计划项目(11TD004)

作者(Author): 高燕;李文婕;李聪颖;范刚;张艺;
GAO Yan;LI Wen-jie;LI Cong-ying;FANG Gang;ZHANG Yi;College of Ethnic Medicine,Chengdu University of Traditional Chinese Medicine;

Email:

DOI:

参考文献(References)：

• [1]中国药典.一部[S].2010:358.
• [2]宇妥·云单贡布.四部医典[M].李永年译.北京:人民卫生出版社,1983.
• [3]晶珠本草[M].毛继祖等译.上海:上海科学出版社,1986.
• [4]庞伟.藏药翼首草的研究与应用[J].中国民族医药杂志,2007(5):63.
• [5]国家药品监督管理局.中药注射剂指纹图谱研究的技术要求(暂行)[J].中成药,2000,22(10):671.
• [6]沈芃.藏药翼首草抗类风湿性关节炎作用与机理研究[D].成都:成都中医药大学,2002.
• [7]张艺.藏药翼首草及其复方然降多吉胶囊的品质评价研究[D].成都:成都中医药大学,2003.
• [8]关昕璐,阎玉凝,魏太明,等.翼首草的抗炎作用与急毒实验研究[J].北京中医药大学学报,2004,27(2):71.