中国中药杂志

2015, v.40(12) 2309-2313

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基于化学分析的不同品种大黄区分研究
Identification of different varieties of Rhei Radix et Rhizoma based on chemical analysis

葛建华;刘训红;许虎;徐丹洋;白发平;
GE Jian-hua;LIU Xun-hong;XU Hu;XU Dan-yang;BAI Fa-ping;Nantong Institute for Drug Control;Nanjing University of Chinese Medicine;Nanjing Haiyuan Chinese Herbal Medicine Co. ,Ltd.;

摘要(Abstract):

该文建立了HPLC对不同品种大黄中5种蒽醌成分以及土大黄苷进行检测,分析比较了不同品种大黄中蒽醌及土大黄苷的含量差异。结果表明,大黄酸与土大黄苷是正品大黄(掌叶组)与非正品大黄(波叶组)区分的指标性成分。正品大黄中未检出土大黄苷,非正品大黄中大黄酸含量痕量。唐古特大黄大黄酸含量较高,通过比较大黄酸与大黄酚含量之间的比值可以将唐古特大黄与另2种正品大黄进行区分。波叶组大黄间土大黄苷含量相差悬殊。
A HPLC method was established to determine the contents of the five anthraquinones and rhaponticin in the different varieties of Rhei Radix et Rhizoma. The difference existed in different varieties. The results showed that rhein and rhaponticin were marker substances which could be used to distinguish palm leaf groups rhubarb and wave leaf groups rhubarb. Authentic rhubarb didn't contain rhaponticin. Falsify rhubarb contains trace amounts of rhein. Rheum tanguticum contains abundant rhein. The ratio value of the content of rhein to chrysophanol could be used to distinguish R. tanguticum from the other two authentic varieties( R. palmatum and R. officinale). The content of rhaponticin varied largely in different varieties of wave leaf groups rhubarb.

关键词(KeyWords): 唐古特大黄;蒽醌;土大黄苷
Rheum tanguticum;anthraquinones;rhaponticin

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基金项目(Foundation): 南京中医药大学医史文献学科项目(WF2013-07)

作者(Author): 葛建华;刘训红;许虎;徐丹洋;白发平;
GE Jian-hua;LIU Xun-hong;XU Hu;XU Dan-yang;BAI Fa-ping;Nantong Institute for Drug Control;Nanjing University of Chinese Medicine;Nanjing Haiyuan Chinese Herbal Medicine Co. ,Ltd.;

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