中国中药杂志

2015, v.40(16) 3158-3167

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接种不同AM真菌对滇重楼菌根侵染率和入药品质的影响
Effects of mycorrhizal colonization and medicine quality of Paris polyphylla var. yunnanensis inoculated by different foreign AM fungi species

周浓;丁博;冯源;戚文华;张华;郭冬琴;向军;
ZHOU Nong;DING Bo;FENG Yuan;QI Wen-hua;ZHANG Hua;GUO Dong-qin;XIANG Jun;College of Pharmacy and Chemistry,Dali University;College of Life Science & Engineering,Chongqing Three Gorges University;

摘要(Abstract):

通过室温盆栽接种试验与室内分析相结合的方法,观察灭菌土壤条件下接种28种丛枝菌根(AM)真菌对药用植物滇重楼根系AM真菌侵染能力和入药质量的影响。结果显示,与对照组(CK)相比,接种外源AM真菌对滇重楼根系AM真菌的孢子密度、侵染率、侵染强度具有调控(增减)作用,增强根内菌丝的琥珀酸脱氢酶(SDH)和碱性磷酸酶(ALP)活性,从而提高滇重楼菌根生活力。AM真菌诱导子处理后,不同AM真菌对滇重楼根茎中不同甾体皂苷的影响有所差异,AM真菌的施用能够提高滇重楼的药用品质,但在实验期间未造成滇重楼甾体皂苷质量的变异。实验结果表明,滇重楼与AM真菌之间的共生关系具有一定的相互选择性,近明球囊霉Claroideoglomus claroideum和瑚状盾巨囊霉Racocetra coralloidea是最适宜滇重楼大田栽培时推广应用的优良接种菌剂。
After 28 foreign species of AM fungi were inoculated in sterilized soil,the effects of the AM mycorrhizal colonization and the medicine quality of Paris polyphylla var. yunnanensis were observed by combination of inoculation test in pot at room temperature and instrumental analysis. The results showed that,compared with control group( CK),the inoculation of foreign AM fungi in the soil influenced the spore density,mycorrhizal infection rate,and colonization intensity of AM fungi in root system of P. polyphylla var.yunnanensis. The inoculation of foreign AM fungi enhanced the mycorrhiza viability of P. polyphylla var. yunnanensis by increasing the activity of succinic dehydrogenase( SDH) and alkaline phosphatase( ALP) in intraradical hyphae. The content of single steroid saponin in rhizome of P. polyphylla var. yunnanensis showed variation after P. polyphylla var. yunnanensis was inoculated by different foreign species of AM fungi,which was beneficial for increasing the medicine quality; however,the kinds of steroid saponin showed no difference. In a degree,there was a selectivity of symbiosis between P. polyphylla var. yunnanensis and foreign AM fungi. And we found that the Claroideoglomus claroideum and Racocetra coralloidea were best foreign AM fungi species for cultivating P. polyphylla var. yunnanensis under field condition.

关键词(KeyWords): 滇重楼;AM真菌;侵染力;琥珀酸脱氢酶;碱性磷酸酶;甾体皂苷;指纹图谱
Paris polyphylla var.yunnanensis;AM fungi;colonization;succinic dehydrogenase;alkaline phosphatase;steroid saponin;fingerprint

Abstract:

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基金项目(Foundation): 国家自然科学基金项目(81260622);; 云南省应用基础研究计划项目(2011FB081);; 云南省教育厅科学研究基金重点项目(2012Z119)

作者(Author): 周浓;丁博;冯源;戚文华;张华;郭冬琴;向军;
ZHOU Nong;DING Bo;FENG Yuan;QI Wen-hua;ZHANG Hua;GUO Dong-qin;XIANG Jun;College of Pharmacy and Chemistry,Dali University;College of Life Science & Engineering,Chongqing Three Gorges University;

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