中国中药杂志

2004, (01)

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复方鳖甲软肝方对肺纤维化大鼠细胞外基质的影响
Influence of compound Biejia Ruangan prescription on extracell uar matrix in bleomycin induced pulmonary fibrosis rats

张东伟,王继峰,牛建昭,杨美娟,王玲巧,金焕,刘连起,高宝华
ZHANG Dong-wei, WANG Ji-feng, NIU Jian-zhao, YANG Mei-juan, W ANG Ling-qiao, JIN Huan, LIU Lian-qi, GAO Bao-hua (Laboratory of Cell and Biochemistry of Beijing University of Chinese Medicin e, Beijing 100029, China)

摘要(Abstract):

目的 :研究复方鳖甲软肝方对博莱霉素诱导大鼠肺纤维化细胞外基质的影响。方法 :SD雄性大鼠 54只 ,分为假手术对照组、模型对照组、复方鳖甲软肝方高剂量组、中、低剂量组、强的松组 ,每组 9只。除假手术对照组外其余各组大鼠气管内一次性滴注盐酸博莱霉素 ,假手术对照组大鼠气管内一次性滴注等体积的生理盐水。 2 8d后给药 ,复方鳖甲软肝方高、中、低剂量组 ,强的松组分别灌服复方鳖甲软肝方 1.4,0 .7,0 .3 5g·kg- 1及强的松 0 .56mg·kg- 1,假手术对照组、模型对照组分别灌服等体积的生理盐水。观察 80d时大鼠肺组织羟脯氨酸和血清中Ⅲ (Ⅲ C) ,Ⅳ (Ⅳ C)胶原及层粘连蛋白 (LN)和透明质酸 (HA)的含量 ,同时取肺组织行HE ,Masson染色。结果 :复方鳖甲软肝方可降低肺纤维化大鼠Ⅰ ,Ⅲ胶原 ,层粘连蛋白及透明质酸含量 ,减轻肺组织纤维性增生。结论 :复方鳖甲软肝方可能是通过降低肺纤维化大鼠细胞外基质含量而发挥治疗肺纤维化作用
Objective: To study the influence of compo un d Biejia Ruangan Prescription(CBRP) on extracelluar matrix in bleomycin induced pulmonary fibrosis rats. Method: 54 male Sprague-Dawley r ats were randomly divided into 6 groups (9 rats in each group). Rats in the mode l control group, positive medicine group, and high, moderate and low CBRP groups were injected with bleomycin A5 by trachea, and rats in sham-model co ntrol group with same volume normal saline. 29 days after the injection, CBRP so lution of different dosages(1.4 g·kg -1 ,0.7 g·kg -1 ,0.35 g·k g -1 ) was respectively given to rats in the high, moderate and low CBRP gro up by gavage, while equal volume of normal saline was given to those in the sham -model control group and model control group, and an equal volume of prednisone (0.56 mg·kg -1 ) was given to those in positive medicine control group. On the 80 th day, the levels of Ⅲ-collagen, Ⅳ-collagen, laminin and hyaluronic acid in the serum were determined, the determination of hydroxyproline in lung h omogenates was analyzed, and the right lung was incised to make pathological sec tions which were stained with Hematoxylin-Eosin(HE) and Masson staining for pat hological diagnosis. Result: CBRP coald decrense the levels of Ⅲ-collagen, Ⅳ-collagen, laminin and hyaluronic acid in the serum. Conclusion: CBRP may play its therapeutic role by levelin g down the content of extracellular matrix in rats with pulmonary fibrosis induc ed by Bleomycin A5.sonstainingforpathologicaldiagnosis .Result :C

关键词(KeyWords): 复方鳖甲软肝方;肺纤维化;Ⅲ型胶原;Ⅳ型胶原;层粘连蛋白;透明质酸
compound Biejia Ruangan prescription(CBRP); rats; pulmonary fibrosis; Ⅲ-collagen; Ⅳ-collagen; laminin; hyaluronic acid

Abstract:

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基金项目(Foundation): 国家自然科学基金资助项目(30 1 30 2 2 0 );; 教育部首批非典科技攻关项目

作者(Author): 张东伟,王继峰,牛建昭,杨美娟,王玲巧,金焕,刘连起,高宝华
ZHANG Dong-wei, WANG Ji-feng, NIU Jian-zhao, YANG Mei-juan, W ANG Ling-qiao, JIN Huan, LIU Lian-qi, GAO Bao-hua (Laboratory of Cell and Biochemistry of Beijing University of Chinese Medicin e, Beijing 100029, China)

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