中国中药杂志

2019, v.44(08) 1607-1614

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基于UPLC-ESI-Q-TOF-MS/MS技术的特色藏族药高原荨麻化学成分快速识别研究
Rapid identification of constituents of Urtica hyperborea using UPLC-ESI-Q-TOF-MS/MS method

苏日娜;罗维早;魏荣锐;奥·乌力吉;钟国跃;
SU Ri-na;LUO Wei-zao;WEI Rong-rui;AO·WU-LI-JI;ZHONG Guo-yue;Research Center for Traditional Chinese Medicine Research and Ethnic Minority Medicine,Jiangxi University of Traditional Chinese Medicine;Chongqing Academy of Chinese Meteria Medica;School of Mongol Medicine,Inner Mongolia University for Nationalities;

摘要(Abstract):

采用超高效液相色谱-串联四极杆飞行时间质谱(UPLC-ESI-Q-TOF-MS/MS)技术对高原荨麻甲醇提取部分化合物进行了快速识别。采用Triple-TOF5600+高分辨质谱仪、超高效液相色谱、YMC-Triart C18色谱柱(2. 1 mm×100 mm,1. 9μm),乙腈-0. 4%甲酸水溶液为流动相梯度洗脱;质谱采用电喷雾(ESI)离子源,在负离子模式下采集数据,通过与对照品图谱中已知成分的保留时间、精确相对分子质量等比对,结合各成分的二级质谱裂解特征比对及文献报道的相同成分或同类成分的质谱碎片裂解规律分析,推测高原荨麻中成分的化学结构。从高原荨麻中初步鉴定出31个化合物,包括黄酮类化合物8个,酚酸类化合物14个,苯丙素类8个(香豆素类4个,木脂素类4个),萜类化合物1个,其中13个成分经对照品比对确认,以上化合物均为首次发现于高原荨麻中,为高原荨麻的药效物质基础等进一步深入研究奠定了基础。
This paper deals with the application of ultra-performance liquid chromatography tandem quadrupole time of flight mass spectrometry(UPLC-ESI-Q-TOF-MS/MS) method to rapidly determine and analyze the chemical constituents of methanol extract of Urtica hyperborea. We employed UPLC YMC-Triart C18(2. 1 mm×100 mm,1. 9 μm) column to UPLC analysis with acetonitrile-water(containing 0. 4% formic acid) in gradient as mobile phase. The flow rate was 0. 3 m L·min-1 gradient elution and column temperature was 30℃; the injection volume was 4 μL. ESI ion source was used to ensure the data collected in anegative ion mode. The chemical components of U. hyperborea were identified through retention time,exact relative molecular mass,cleavage fragments of MS/MS and reported data.The results indicated that a total of 31 compounds were identified,including 8 flavonoids,14 phenolic compounds,8 phenylpropanoids(4 coumarins and 4 lignans),and 1 steroidal compound,13 of which were confirmed by comparison. The UPLC-ESI-Q-TOF-MS/MS method could rapid identify the chemical components of U. hyperborea. The above compounds were discovered in U. hyperborea for the first time,which could provide theoretical foundation for further research on the basis of the pharmacodynamics of U. hyperborea.

关键词(KeyWords): 高原荨麻;UPLC-ESI-Q-TOF-MS/MS;化学成分;快速鉴别
Urtica hyperborea;UPLC-ESI-Q-TOF-MS/MS;chemical composition;rapid identification

Abstract:

Keywords:

基金项目(Foundation): 江西民族传统药现代科技与产业发展协同创新中心项目(JXXT2017009);; 江西中医药大学双一流项目(JXSYLXK-ZHYAO034)

作者(Author): 苏日娜;罗维早;魏荣锐;奥·乌力吉;钟国跃;
SU Ri-na;LUO Wei-zao;WEI Rong-rui;AO·WU-LI-JI;ZHONG Guo-yue;Research Center for Traditional Chinese Medicine Research and Ethnic Minority Medicine,Jiangxi University of Traditional Chinese Medicine;Chongqing Academy of Chinese Meteria Medica;School of Mongol Medicine,Inner Mongolia University for Nationalities;

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DOI: 10.19540/j.cnki.cjcmm.20190118.005

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