中国中药杂志

2018, v.43(03) 469-477

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光照对药食同源植物苦荞麦种子中原花青素积累关键酶FtANR,FtLAR基因表达的影响
Influence of light on gene expression of key synthesis enzyme genes FtANR and FtLAR about proanthocyanidin in seeds of homologous plant of food and medicine Fagopyrum tataricum

姜春丽;苏新堯;许亚春;吴而已;师玉华;张栋;陈庆富;孙伟;薛建平;
JIANG Chun-li;SU Xin-yao;XU Ya-chun;WU Er-yi;SHI Yu-hua;ZHANG Dong;CHEN Qing-fu;SUN Wei;XUE Jian-ping;College of Life Sciences,Huaibei Normal University;Key Laboratory of Beijing for Identification and Safe Evaluation of Chinese Medicine,Institute of Chinese Meteria Medica,China Academy of Chinese Medical Sciences;College of Life Science,Northwest Agriculture & Forestry Unversity;Guizhou Normal University;

摘要(Abstract):

苦荞麦Fagopyrum tataricum作为重要的药食两用植物,由于种子中含有丰富的黄酮类成分,因而具有良好的清除自由基、抗氧化、抗衰老等作用。现有的苦荞麦黄酮类化合物研究大多集中在黄酮醇类化合物如芦丁的合成、调控以及积累上,但针对苦荞麦中原花青素类成分的代谢途径研究鲜有报道。该研究以原花青素代谢途径中关键的合成基因花青素还原酶(ANR)和原花青素还原酶(LAR)入手,通过挖掘苦荞麦转录组信息克隆了Ft ANR(F.tataricum ANR),Ft LAR1(F.tataricum LAR1),Ft LAR3(F.tataricum LAR3)。由于光在植物次生代谢物质形成与积累过程中发挥着重要作用,该研究试着探索不同光质(红光、蓝光、远红光、紫外光)对原花青素代谢途径中关键的合成基因ANR,LAR的表达影响。该研究发现红光对所克隆基因表达的影响呈现出与其他光相反的作用:Ft ANR,Ft LAR1在红光处理后基因的表达量有所下降,而其在蓝光、远红光、紫外光处理后基因的表达量是有所上调的;Ft LAR3在红光处理后基因的表达量有所上调,而其在蓝光、远红光、紫外光处理后基因的表达量是有所下降的。
Tartary buckwheat Fagopyrum tataricum is an important medicinal and functional herb due to its rich content of flavonoids in the seeds. F. tataricum exhibited good functions for free radicals scavenging,anti-oxidation,anti-aging activities. Although much genetic knowledge of the synthesis,regulation,accumulation of rutin,the genetic basis of proanthocyanidins( PAs) in tartary buckwheat and their related gene expression changes under different lights( blue,red,far red,ultraviolet light) remain largely unexplored. In this study,we cloned one anthocyanidin reductase gene( ANR) and two leucocyanidin reductase gene( LAR) named Ft ANR,FtLAR1,FtLAR3 involved in formation of( +)-catechin and(-)-epicatechin precusor proanthocyanidin by digging out F. tataricum seed transcriptome data. The expression data showed that the opposite influence of red light on these gene transcript level compared to others lights. The expression levels of Ft ANR and FtLAR1 decreased and FtLAR3 appeared increment after exposed in the red light,while the expression levels of those genes appeared opposite result after exposed in the blue and far red light.

关键词(KeyWords): 苦荞麦;原花青素;光诱导;基因表达;定量分析
Fagopyrum tataricum;proanthocyanidin;light induction;gene expression;quantitative analysis

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81573518,81603248);; 安徽省自然科学基金项目(1408085MC58);; 中央级公益性科研院所基本科研业务费专项(GH2017-01-01,GH2017-01)

作者(Author): 姜春丽;苏新堯;许亚春;吴而已;师玉华;张栋;陈庆富;孙伟;薛建平;
JIANG Chun-li;SU Xin-yao;XU Ya-chun;WU Er-yi;SHI Yu-hua;ZHANG Dong;CHEN Qing-fu;SUN Wei;XUE Jian-ping;College of Life Sciences,Huaibei Normal University;Key Laboratory of Beijing for Identification and Safe Evaluation of Chinese Medicine,Institute of Chinese Meteria Medica,China Academy of Chinese Medical Sciences;College of Life Science,Northwest Agriculture & Forestry Unversity;Guizhou Normal University;

Email:

DOI: 10.19540/j.cnki.cjcmm.20180105.005

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