中国中药杂志

2008, v.33(24) 2932-2935

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秦皮药材HPLC指纹图谱研究
Study on fingerprint of Cortex Fraxini with HPLC

刘丽梅;陈琳;王瑞海;杨庆;翁小刚;王丽;
LIU Li-mei1,CHEN Lin1,WANG Rui-hai1,YANG Qing2,WENG Xiao-gang2,WANG Li3(1.Basic Theory Research Institute of Chinese Medicine,China Academy of Chinese Medical Sciences,Beijing 100700,China;2.Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;3.Sanjing Pharmaceutical Co.Ltd..Harbin Pharmaceutical Group,Harbin 150069,China)

摘要(Abstract):

目的:建立秦皮药材的指纹图谱,为科学评价秦皮质量提供参考依据。方法:采用高效液相色谱技术,Agilent Extend C18柱(4.6 mm×250 mm,5μm),甲醇-0.4%醋酸水溶液梯度洗脱,检测波长0~60 min,340 nm;60~74 min,254 nm;74~75 min,340 nm,柱温40℃,流速1.5 mL.min-1。4个品种药材各测定10批,分别建立指纹图谱。结果:指纹图谱研究的各项参数均符合有关规定,苦枥白蜡树10批药材有15个共有峰,尖叶白蜡树10批药材有11个共有峰,白蜡树10批药材有11个共有峰,宿柱白蜡树10批药材有19个共有峰,秦皮药材40批药材有5个共有峰,与对照图谱相比较,每个品种药材指纹图谱相似度均在0.96以上。40批秦皮药材指纹图谱相似度大于0.90,40批药材中检测出4个有效成分(秦皮甲素,秦皮乙素,秦皮苷,秦皮素)及1个未知成分。结论:建立了苦枥白蜡树树皮,白蜡树树皮,尖叶白蜡树树皮,宿柱白蜡树树皮指纹图谱及秦皮药材的指纹图谱,方法学考察结果符合指纹图谱技术要求。
Objective:To establish fingerprint of Cortex Fraxini and provide reference for quality evaluation of Cortex Fraxini.Method: Chromatographic experiments were performed on Agilent Extend C18 column(4.6 mm×250 mm,5 μm),eluted with methanol and water,containing 0.4% acetic acid as the mobile phases in gradient elution.The detection wavelength was 0-60 min,340 nm;6-74 min,254 nm;74-75 min,340 nm,and the flow rate was 1.0 mL·min-1.Forty samples in four varieties of Cortex Fraxini were detected to establish fingerprints,respectively.Result: Every parameter of the method validation complied with related rules and regulations.There were 15 common peaks in the fingerprint of 10 Fraxinus rhynchophylla samples,eleven common peaks in the fingerprint of 10 F.chinensis var.acuminata.samples,and in the fingerprint of 10 F.chinensis samples.Nineteen common peaks in the fingerprint of 10 F.stylosa samples.There were 5 common peaks in the fingerprints of 40 Cortex Fraxini samples.The similarity factors of the 10 samples of every species were all more than 0.96 compared with the control fingerprint.The similarity of the 40 Cortex Fraxini samples was more than 0.90.Four effective constituents and one unknown constituent were found in 40 samples.Conclusion: The fingerprints of F.rhynchophylla bark,F.chinensis bark,F.chinensis var.acuminata bark,F.stylosa bark and Cortex Fraxini were established.The methodological evaluation showed that the results were in accord with the technology requirements of chromatography fingerprint,and it laid a good foundation for quality control of Cortex Fraxini.

关键词(KeyWords): 秦皮药材;HPLC;指纹图谱
Cortex Fraxini;HPLC;fingerprint

Abstract:

Keywords:

基金项目(Foundation): 北京市自然科学基金项目(706308)

作者(Author): 刘丽梅;陈琳;王瑞海;杨庆;翁小刚;王丽;
LIU Li-mei1,CHEN Lin1,WANG Rui-hai1,YANG Qing2,WENG Xiao-gang2,WANG Li3(1.Basic Theory Research Institute of Chinese Medicine,China Academy of Chinese Medical Sciences,Beijing 100700,China;2.Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;3.Sanjing Pharmaceutical Co.Ltd..Harbin Pharmaceutical Group,Harbin 150069,China)

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