李红娟;李家春;胡军华;刘威;毕宇安;王振中;萧伟;
LI Hong-juan;LI Jia-chun;HU Jun-hua;LIU Wei;BI Yu-an;WANG Zhen-zhong;XIAO Wei;Jiangsu Kanion Pharmaceutical Co. Ltd;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process;School of Pharmacy,Liaoning University of Traditional Chinese Medicine;

• 1:江苏康缘药业股份有限公司
• 2:中药制药过程新技术国家重点实验室
• 3:辽宁中医药大学药学院

摘要(Abstract)：

目的:建立并比较茯苓不同药用部位中三萜酸类成分的HPLC特征指纹图谱,为完善茯苓的质量控制方法提供依据。方法:采用HPLC测定,Waters Symmetry C18色谱柱(4.6 mm×250 mm,5μm);流动相乙腈-0.1%磷酸溶液,梯度洗脱;检测波长210 nm;体积流量1.0 m L·min-1;柱温30℃;采用SPSS 15.0进行聚类分析。结果:建立了茯苓不同药用部位中三萜酸类成分的指纹图谱;22批茯苓药材指纹图谱中标示出16个共有峰,方法学考察结果符合指纹图谱技术要求;相似度和聚类分析结果表明,白茯苓、茯神与赤茯苓中三萜酸成分存在显著差异,赤茯苓中茯苓酸的含量最高。药材的质量产地间无显著差异。结论:该方法稳定可靠,重复性好,所建立的指纹图谱能有效的评价茯苓药材的质量。
Objective: To establish an analytical method for the fingerprint of triterpenoid constituents of Poria by HPLC and compare the fingerprints of different medicinal parts of Poria in order to provide basis for controlling Poria quality. Method: The HPLC chromatographic conditions were Waters Symmetry C18column(4. 6 mm × 250 mm,5 μm),0. 1% phosphoric acid(A) and acetonitrile(B) as gradient mobile phases,flow rate being 1. 0 m L·min- 1,column temperature at 30 ℃,The detection wavelength was set at 210 nm; The cluster analysis was carried on by SPSS 15. 0. Result: The HPLC fingerprints of triterpenoid constituents of Poria were set up. There were 16 common peaks in different medicinal parts. The results of method validation met technical requirement of fingerprints; Triterpenoid constituents in White Poria and Poria cum Radix Pini were different from Poria. The content of pachymic acid was the highest in Poria. The effect of habitat on the quality was no obvious difference. Conclusion: The method is stable,reliable,reproducible,and can be used as an effective means of Poria quality evaluation.

关键词(KeyWords)： 茯苓;HPLC指纹图谱;聚类分析;相似度
Poria;HPLC fingerprint;cluster analysis;similarity

Abstract:

Keywords:

基金项目(Foundation): 国家“重大新药创制”科技重大专项(2011ZX09101403)

作者(Author): 李红娟;李家春;胡军华;刘威;毕宇安;王振中;萧伟;
LI Hong-juan;LI Jia-chun;HU Jun-hua;LIU Wei;BI Yu-an;WANG Zhen-zhong;XIAO Wei;Jiangsu Kanion Pharmaceutical Co. Ltd;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process;School of Pharmacy,Liaoning University of Traditional Chinese Medicine;

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DOI:

参考文献(References)：

• [1]中国药典.一部[S].2010:224.
• [2]蔡光先,谢昭明,黄丹,等.不同产地茯苓中茯苓多糖的比较研究[J].湖南中医杂志,2012,28(1):125.
• [3]胡明华,梁永威,彭川丛.不同产地不同规格的茯苓水溶性多糖含量比较[J].中国药业,2012,21(7):10.
• [4]胡斌,杨益平,叶阳.茯苓化学成分研究[J].中草药,2006,37(5):655.
• [5]昝俊峰,徐斌,於小波,等.全国20个主要产地茯苓质量分析比较研究[J].中国中医药信息杂志,2010,17(8):24.
• [6]许甜甜,金传山,吴德玲,等.茯苓不同药用部位化学成分分析[J].安徽中医学院学报,2013,32(1):77.
• [7]段启,王跃生,王少军,等.不同产地茯苓药材高效液相色谱指纹图谱的实验研究[J].中国实验方剂学杂志,2006,7(12):11.
• [8]昝俊峰,徐斌,刘军锋,等.20个产地茯苓三萜成分RPHPLC-ELSD指纹图谱[J].中国实验方剂学杂志,2013,19(21):65.
• [9]昝俊峰,徐斌,刘军锋,等.茯苓三萜成分RP-HPLC-ELSD指纹图谱研究[J].中草药,2012,43(5):895.
• [10]张琦,王振中,萧伟,等.茯苓UPLC特征指纹图谱[J].中国中药杂志,2012,37(7):966.
• [11]丁岗,王振中,章晨峰,等.茯苓中三萜酸类成分HPLC指纹图谱的初步研究[J].中国中药杂志,2002,27(10):756.
• [12]李珂,张立群,聂晶.不同药用部位茯苓UPLC-UV-MS指纹图谱比较研究[J].中药材,2013,3(36):382.
• [13]仲兆金,许先栋.茯苓三萜化学成分及其光谱特征研究进展[J].中国药物化学杂志,1997,7(1):71.
• [14]仲兆金,刘浚.茯苓有效成分三萜的研究进展[J].中成药,2001,23(1):58.