中国中药杂志

2014, v.39(05) 795-802

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铁皮石斛茎、叶多糖含量及多糖部位柱前衍生化-高效液相色谱指纹图谱比较研究
Comparison on polysaccharide content and PMP-HPLC fingerprints of polysaccharide in stems and leaves of Dendrobium officinale

周桂芬;庞敏霞;陈素红;吕圭源;颜美秋;
ZHOU Gui-fen;PANG Min-xia;CHEN Su-hong;LV Gui-yuan;YAN Mei-qiu;Zhejiang Chinese Medical University;Wenzhou Medical University;

摘要(Abstract):

为更好地开发利用铁皮石斛叶资源,该文采用苯酚-硫酸比色法和PMP(1-苯基-3-甲基-5-吡唑啉酮)柱前衍生化-HPLC-DAD-ESI-MSn对铁皮石斛茎、叶中多糖含量、单糖组成、多糖中甘露糖和葡萄糖的含量及多糖部位HPLC指纹图谱进行了比较研究;并用国家药典委员会"中药色谱指纹图谱相似度评价软件2004A"建立了对照图谱和进行了相似度评价。结果表明,铁皮石斛茎与叶多糖含量,组成多糖的单糖种类,各单糖组成比例及单糖含量明显不同。叶中多糖含量约为相应茎中多糖含量的1/3。茎多糖主要由甘露糖和葡萄糖组成;叶多糖为酸性杂多糖,由甘露糖、半乳糖醛酸、葡萄糖、半乳糖和阿拉伯糖组成,但茎和叶多糖中均以甘露糖组成比例最高。茎中甘露糖和葡萄糖的含量均比相应叶中高。14个不同种植基地铁皮石斛茎和叶多糖部位指纹图谱相似性较好,相似度均在0.9以上。该文为进一步研究铁皮石斛叶多糖中各单糖连接顺序、连接位置和多糖药理活性及申报铁皮石斛叶新资源提供了研究资料。
In order to provide scientific basics for exploitation and sufficient application of Dendrobium officinale leaves resources,the phenol-sulfuric acid method was applied to determine the polysaccharide content. The monosaccharides were derivated by PMP and the derivatives were identified by HPLC-DAD-ESI-MSnand the contents of mannose and glucose were determined simultaneously. Similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine( 2004A) was employed to generate the mean chromatogram and similarity analysis of the samples was carried out. The results demonstrated that polysaccharide content, monosaccharide compositions and composition ratio had an obvious difference between stems and leaves. The polysaccharide content of stems was higher than that of leaves. Monosaccharide composition in leaf was significantly different from that in stem. The polysaccharide from stems was composed of mannose and glucose,however the polysaccharide of leaves was acid heteropolysaccharide and was mainly composed of five monosaccharides,including mannose,galacturonic acid,glucose,galactose and arabinose. The similarity value of the 14 batches was above 0. 9,indicating that similarity of fingerprints among different samples was high. The study can provide evidence for expanding the medicinal parts of D. officinale.

关键词(KeyWords): 铁皮石斛;茎;叶;多糖;指纹图谱;甘露糖;葡萄糖
Dendrobium officinale;stem;leaf;polysaccharide;fingerprints;mannose;glucose

Abstract:

Keywords:

基金项目(Foundation): 国家“十二五”科技支撑计划项目(2011BA104B06);; 浙江省教育厅科研项目(Y201328964);; 浙江省重大科技专项重点项目(2006C13014)

作者(Author): 周桂芬;庞敏霞;陈素红;吕圭源;颜美秋;
ZHOU Gui-fen;PANG Min-xia;CHEN Su-hong;LV Gui-yuan;YAN Mei-qiu;Zhejiang Chinese Medical University;Wenzhou Medical University;

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