中国中药杂志

2020, v.45(24) 5944-5950

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光照强度对紫花地丁物质和能量代谢的影响
Effects of light intensity on material and energy metabolism of Viola yedoensis

杨培芳;史红专;郭巧生;王羿;禤汉美;刘丽;赵婵婵;
YANG Pei-fang;SHI Hong-zhuan;GUO Qiao-sheng;WANG Yi;XUAN Han-mei;LIU Li;ZHAO Chan-chan;Institute of Chinese Medicinal Materials, Nanjing Agricultural University;

摘要(Abstract):

光是影响植物生长的重要因素,"光照-寒热药性"假说认为寒性中药其基原植物在强光环境中能获取更多的能量维持生长,反之热性中药其基原植物喜欢弱光环境。该实验研究不同光强对寒性中药紫花地丁基原植物紫花地丁初级代谢物质、能量水平、细胞凋亡及叶片结构等方面的影响。在Li1(8 500 lx),Li2(7 250 lx),Li3(6 000 lx),Li4(4 750 lx),Li5(3 500 lx)5个LED光强下种植紫花地丁,采收期测定初级代谢物质,ATP,ADP,AMP含量,ATP合成和水解相关酶活性,细胞凋亡活化程度,并利用透射电镜观察叶片超微结构。结果表明,紫花地丁总糖,总蛋白,ATP,ADP,AMP含量,NADH脱氢酶,细胞色素C还原酶,ATP合酶和ATP水解酶的活性与光强呈正相关(P<0.05);粗脂肪含量,SDH,CCO酶活呈先增后减的趋势,分别在Li2, Li3组最高(P<0.05); caspase-3,caspase-9活力与光强呈负相关(P<0.05); Li1~Li3组叶绿体和线粒体结构正常且完整,Li4,Li5组结构受损程度随光强减弱而增强。综上,紫花地丁在6 000~8 500 lx光强下叶绿体和线粒体结构正常,能获取更多能量维持其生长和代谢,光强低于4 750 lx时叶绿体形态和线粒体膜出现损坏,影响物质和能量代谢,3 500~8 500 lx光强范围紫花地丁能荷无差异。光强对紫花地丁能量代谢的影响符合"光照-寒热药性"假说。
Light energy is an important factor affecting plant growth. The hypothesis of "light-cold and heat property" holds that the original plants of traditional Chinese medicines(TCM) with cold property can obtain more energy to maintain growth in high light intensity environment, whereas the original plants of TCM with heat property prefer weak light environment. The current experiment investigated the effects of different light intensities on primary metabolites levels, energy levels, cell apoptosis, and leaves ultrastructure of Viola yedoensis, the original plants of TCM Violae Herba with cold property. There were five treatment groups of V. yedoensis, which was planted under Li1(8 500 lx),Li2(7 250 lx),Li3(6 000 lx),Li4(4 750 lx),Li5(3 500 lx)LEDs light intensity conditions, respectively. After harvest, primary metabolites levels, contents of ATP, ADP, AMP, activities of ATP synthesis and hydrolysis related enzyme, as well as cell apoptosis activation degree were measured, and transmission electron microscopy technique was used to observe leaves ultrastructure. The results showed that the total sugar, total protein, contents of ATP, ADP and AMP, activities of NADH dehydrogenase, cytochrome C reductase, ATP synthase and ATP hydrolase were positively correlated with light intensities(P<0.05). The crude fat content, activities of SDH and CCO enzyme showed a trend of increasing first and then decreasing, the highest value were found in Li2 group and Li3 group respectively(P<0.05). The vitality of caspase-3 and caspase-9 was negatively correlated with light intensities(P<0.05). The structure of chloroplast and mitochondria were normal and intact in Li1-Li3 groups, and the damage degree of Li4 and Li5 groups increased with the decrease of light intensities. The analysis of results indicated that the structure of chloroplast and mitochondria of V. yedoensis is normal under the light intensity of 6 000-8 500 lx, which can obtain more energy to maintain its growth and metabolism. When the light intensity is lower than 4 750 lx, the chloroplast morphology and mitochondrial membrane are damaged, affecting the metabolism of material and energy. There was no significant difference in energy charge of V. yedoensis in the light intensity range of 3 500~8 500 lx. The effect of light intensity on energy metabolism of V. yedoensis accords with the hypothesis of "light-cold and heat property".

关键词(KeyWords): 光照强度;紫花地丁;“光照-寒热药性”假说;线粒体;能量代谢
light intensity;Viola yedoensis;"light-cold and heat property" hypothesis;mitochondria;energy metabolism

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(41471243)

作者(Author): 杨培芳;史红专;郭巧生;王羿;禤汉美;刘丽;赵婵婵;
YANG Pei-fang;SHI Hong-zhuan;GUO Qiao-sheng;WANG Yi;XUAN Han-mei;LIU Li;ZHAO Chan-chan;Institute of Chinese Medicinal Materials, Nanjing Agricultural University;

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DOI: 10.19540/j.cnki.cjcmm.20200927.102

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