中国中药杂志

2014, v.39(11) 2056-2059

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炙甘草药材指纹图谱研究
HPLC fingerprint of Glycyrrhizea Radix et Rhizoma Praeparata Cum Melle

孙磊;靳勇;刘晓晴;乔善义;高松;车彦忠;
SUN Lei;JIN Yong;LIU Xiao-qing;QIAO Shan-yi;GAO Song;CHE Yan-zhong;Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences;School of Pharmaceutical College,Central South University;Henan Wanxi Pharmaceutical Co.,Ltd.;

摘要(Abstract):

采用Agilent TC-C18(2)色谱柱(4.6 mm×250 mm,5μm),以乙腈-水(含0.2%甲酸)为流动相梯度洗脱建立了炙甘草药材的HPLC指纹图谱,并采用HPLC-MS/MS对19个共有峰中的6个色谱峰进行了定性鉴别。采用该方法测定了10批不同产地的炙甘草药材,其相似度在0.72~0.99。该方法精密度、稳定性和重复性良好,该研究为炙甘草药材的质量控制提供了参考。
The chromatographic fingerprint was established by eluting with the mobile phase consisted of acetonitrile and0.2%formic acid water on an Agilent TC-C18(2) column(4.6 mm ×250 mm,5 μm).Six chromatographic peaks were identified by HPLCMS/MS method.Ten batches of Glycyrrhizea Radix et Rhizoma Praeparata Cum Melle were determined,and the similarity was arranged from 0.72 to 0.99.Good precision,stability and repeatability were obtained,and this study provides a reference for the quality control of Glycyrrhizea Radix et Rhizoma Praeparata Cum Melle.

关键词(KeyWords): 炙甘草;HPLC;指纹图谱;质量控制
Glycyrrhizea Radix et Rhizoma Praeparata Cum Melle;HPLC;fingerprint;quality control

Abstract:

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基金项目(Foundation): 国家“重大新药创制”科技重大专项(2011ZX09201-201-13,2012ZX09301003-001-004,2013ZX09508-105)

作者(Author): 孙磊;靳勇;刘晓晴;乔善义;高松;车彦忠;
SUN Lei;JIN Yong;LIU Xiao-qing;QIAO Shan-yi;GAO Song;CHE Yan-zhong;Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences;School of Pharmaceutical College,Central South University;Henan Wanxi Pharmaceutical Co.,Ltd.;

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