中国中药杂志

2020, v.45(14) 3452-3458

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霍山石斛茎化学成分及其抗炎活性研究
Study on chemical constituents of Dendrobium huoshanense stems and their anti-inflammatory activity

苏双巧;江慧;李强明;查学强;罗建平;
SU Shuang-qiao;JIANG Hui;LI Qiang-ming;ZHA Xue-qiang;LUO Jian-ping;School of Food and Biological Engineering, Hefei University of Technology;

摘要(Abstract):

该实验从霍山石斛茎中分离得到3个联苄类化合物和6个其他化合物,并鉴定其为3′,4-二羟基-3,5′-二甲氧基联苄(1),山药素Ⅲ(2),3,4′-二羟基-5-甲氧基联苄(3),二氢松柏醇二氢对羟基桂皮酸酯(4),丁香树脂酚(5),3-(4-羟苯基)-丙酸乙酯(6),(3-乙基苯基)-1,2-乙二醇(7),(S)-5-羟基-3,4-甲基-5-正戊基呋喃-2(5氢)-酮(8)和地芰普内酯(9),化合物1~9均是首次从霍山石斛茎中分离得到。抗炎活性测试结果显示联苄类化合物1~3对LPS诱导巨噬细胞RAW264.7的一氧化氮(NO)产生以及肿瘤坏死因子α(TNF-α)和白细胞介素1β(IL-1β)mRNA表达具有显著抑制作用,说明联苄类化合物1~3具有显著的抗炎活性。抗炎作用机制研究显示化合物1能够抑制LPS诱导巨噬细胞RAW264.7引起的核因子κB(NF-κB)p65,NF-κB抑制蛋白(IκB),细胞外调节蛋白激酶(ERK),c-Jun氨基末端激酶(JNK),p38和蛋白激酶B(Akt)的磷酸化,说明化合物1可能是通过抑制NF-κB、丝裂原活化蛋白激酶(MAPKs)和Akt信号通路发挥抗炎作用。
Three bibenzyls 1-3 and six other compounds 4-9 were firstly isolated from Dendrobium huoshanense stems. They were identified as 3′,4-dihydroxy-3,5′-dimethoxybibenzyl(1), batatasin Ⅲ(2), 3,4′-dihydroxy-5-methoxy bibenzyl(3), dihydroconiferyl dihydro-p-coumarate(4), syringaresinol(5), 3-(4-hydroxyphenyl)-propionic acid ethyl ester(6),(3-ethylphenyl)-1,2-ethanediol(7),(S)-5-hydroxy-3,4-dimethyl-5-pentylfuran-2(5H)-one(8) and loliolide(9). Anti-inflammation assay showed that bibenzyls 1-3 could significantly inhibit the production of nitric oxide(NO) and the expression of tumor necrosis factor α(TNF-α) and interleukin 1β(IL-1β) mRNA in LPS-induced RAW264.7 macrophages. Mechanism study exhibited that the phosphorylation of nuclear factor kappa B(NF-κB) p65, inhibitor of κB(IκB), extracellular regulatedprotein kinase(ERK), c-Jun N-terminalkinase(JNK), p38 and Akt of LPS-induced RAW264.7 macrophages could be remarkably reduced by 1. These results suggested that the inflammatory response of LPS-induced RAW264.7 macrophages could be significantly inhibited by 1-3. Additionally, the anti-inflammatory effect of 1 might be contributed to its ability on the regulation of NF-κB, MAPKs and Akt signaling pathways.

关键词(KeyWords): 霍山石斛;化学成分;联苄;抗炎活性
Dendrobium huoshanense;chemical constituents;bibenzyl;anti-inflammation

Abstract:

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基金项目(Foundation): 国家自然科学基金项目(21702040,31970372);; 中国博士后科学基金项目(2017M612069);; 中央高校基本科研业务费专项资金项目(JZ2019HGTB0065)

作者(Author): 苏双巧;江慧;李强明;查学强;罗建平;
SU Shuang-qiao;JIANG Hui;LI Qiang-ming;ZHA Xue-qiang;LUO Jian-ping;School of Food and Biological Engineering, Hefei University of Technology;

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