姚令文;石岩;孙冬梅;程显隆;魏锋;马双成;
YAO Ling-wen;SHI Yan;SUN Dong-mei;CHENG Xian-long;WEI Feng;MA Shuang-cheng;National Institutes for Food and Drug Control;Jilin Institute for Food and Drug Control;

• 1:中国食品药品检定研究院
• 2:吉林市食品药品检验所

摘要(Abstract)：

建立了人工牛黄薄层指纹图谱,对其图像进行数字化和化学计量学研究。首先采用以环己烷-乙酸乙酯-乙酸-甲醇(2∶7∶1∶2)为展开剂,10%硫酸乙醇溶液为显色剂,105℃加热至斑点清晰,并在366 nm下采集数码图像。然后将该图像转换成为灰度图像,得到指纹图谱的灰度曲线图多元数据,并采用主成分分析法对多元数据进行分析。结果表明薄层指纹图谱可表征不同生产企业生产的人工牛黄,并且分析得到图谱上表征来源的特征区域。该方法简便,快速,适合用于不同来源的人工牛黄质量差异的评价。
A method of thin-layer fingerprinting chromatogram of artificial cow-bezoar was established with the developing solvent consisting of cyclohexane,ethyl acetate,acetic acid and methanol( 2 ∶ 7 ∶ 1 ∶ 2),and 10 % sulfuric acid ethanol solution sprayed as colour-developing agent. After heated at 105 ℃,TLC was recorded as an image in ultraviolet light at 366 nm which was converted into grayscale. By the gray value extracted from the grayscale,the multivariate data obtained from TLC of samples could be analyzed by chemometric method. The results indicated that samples from different manufacturers could be distinguished by this method and some specific bands were found out. All in one,this simple and practical method was suitable for the evaluation of quality difference.

关键词(KeyWords)： 人工牛黄;薄层色谱;指纹图谱;胆汁酸;化学计量学;主成分分析
artificial cow-bezoar;TLC;fingerprinting;bile acids;chemometrics;principle component analysis

Abstract:

Keywords:

基金项目(Foundation): 国家“重大新药创制”科技重大专项(2014ZX09304307-002)

作者(Author): 姚令文;石岩;孙冬梅;程显隆;魏锋;马双成;
YAO Ling-wen;SHI Yan;SUN Dong-mei;CHENG Xian-long;WEI Feng;MA Shuang-cheng;National Institutes for Food and Drug Control;Jilin Institute for Food and Drug Control;

Email:

DOI: 10.19540/j.cnki.cjcmm.20170223.026

参考文献(References)：

• [1]国家食品药品监督管理总局.数据速查[EB/OL].[2017-01-03].http://www.sda.gov.cn/WS01/CL0001/.
• [2]中国药典.一部[S].2015:7.
• [3]罗晓茹,曹红,邢俊波,等.人工牛黄及其制剂中胆酸含量测定的研究进展[J].解放军药学学报,2009,25(3):247.
• [4]罗珍妹,陈晓玲.高效液相色谱法测定人工牛黄中胆红素的含量[J].海峡药学,2010,22(6):48.
• [5]徐丽婷,丁静慧,孙泰俊,等.高效液相色谱法测定人工牛黄药材及制剂中胆红素含量[J].医药导报,2004,23(1):49.
• [6]刘力丰.紫外分光光度法测定牛黄至宝丸中胆红素的含量[J].中国实验方剂学杂志,2010,16(16):62.
• [7]叶蓓蓓,潘莉,王栋,等.薄层扫描法同时测定人工牛黄中胆酸及猪去氧胆酸的含量[J].药物分析杂志,2010,30(4):706.
• [8]王伟毅,夏晶,季申.牛黄抱龙片中人工牛黄含量测定方法的研究[J].中成药,2010,32(3):436.
• [9]李文春,孙永慧.HPLC-ELSD法测定青羚散中胆酸的含量[J].中国实验方剂学杂志,2005,11(5):18.
• [10]薛纯盛,栗平.人工牛黄中胆酸比色定量法改进[J].中国药事,2000,14(1):43.
• [11]刘苇,金城,孔维军,等.高效液相色谱-蒸发光散射法测定牛黄中多种有效成分含量及其质量相关性研究[J].分析化学,2010,38(4):522.
• [12]郭丙炎,胡翮,邱蓓.HPLC法测定氨咖黄敏胶囊中人工牛黄的含量[J].药物分析杂志,2009,29(8):1367.
• [13]熊婧,郑天骄,石岩,等.人工牛黄甲硝唑胶囊中主要胆汁酸类成分HPLC-ELSD法测定研究[J].药物分析杂志,2015,35(6):1067.
• [14]中国药典.四部[S].2015:58.
• [15]Nielsen N P V,Carstensen J M,Smedsgaard J.Aligning of single and multiple wavelength chromatographic profiles for chemometric data analysis using correlation optimised warping[J].J Chromatogr A,1998,805(1/2):17.
• [16]Zheng L,Watson D G,Johnston B F,et al.A chemometric study of chromatograms of tea extracts by correlation optimization warping in conjunction with PCA,support vector machines and random forest data modeling[J].Anal Chim Acta,2009,642(1/2):257.
• [17]Ropkins K,Carlsaw D C,Goodman P S,et al.Application of non-linear time-alignment and integration methods to environmental time series[J].Trends Anal Chem,2009,28(3):373.
• [18]Shi Y,Xiong J,Sun,D M,et al.Simultaneous quantification of the major bile acids in Artificial Calculus bovis by high-performance liquid chromatography with precolumn derivatization and its application in quality control[J].J Sep Sci,2015,38(16):2753.
• [19]石岩,孙冬梅,魏锋,等.体外培育牛黄中主要胆酸类成分的测定[J].药物分析杂志,2013,33(6):1045.
• [20]石岩,魏锋,林瑞超,等.熊胆粉中主要胆汁酸类成分的测定及分析[J].中国药学杂志,2016,51(22):1958.
• [21]石岩,郑天骄,魏锋,等.牛胆粉指纹图谱模式识别及多组分测定研究[J].中国中药杂志,2016,41(13):2487.