中国中药杂志

2004, (02)

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铁皮石斛降血糖作用及其机制的研究
Studies on anti-hyperglycemic effect and its mechanism of Dendrobium candidum

吴昊姝,徐建华,陈立钻,孙继军
WU Hao-shu 1,XU Jian-hua 1,CHEN Li-zuan 2,SUN Ji-jun 2 ( 1.Department of Pharmacology and Toxicology,Collage of Pharmaceutical Sciences,Zhejiang University,Hangzhou 310031,China; 2.Zhejiang TianHuang Pharmaceutical CO.LTD. Hangzhou 310012,China )

摘要(Abstract):

目的 :研究铁皮石斛的降血糖作用及其机制。方法 :采用正常小鼠 ,肾上腺素性高血糖小鼠 ,链脲佐菌素性糖尿病 (STZ DM)大鼠 ,用放射免疫分析和免疫组化HRP SPA染色等方法进行研究。结果 :铁皮石斛对正常小鼠血糖及血清胰岛素水平无明显影响 ,但可使STZ DM大鼠的血糖值降低、血清胰岛素水平升高、胰高血糖素水平降低。免疫组化染色显示 ,给药大鼠胰岛 β细胞数量增多 ,α细胞数量减少。它还可使肾上腺素性高血糖小鼠血糖降低、肝糖原含量增高。结论 :铁皮石斛对肾上腺素性高血糖小鼠及STZ DM大鼠有明显的降血糖作用。其降血糖的胰内机制是促进胰岛 β细胞分泌胰岛素 ,抑制胰岛α细胞分泌胰高血糖素 ,胰外机制可能是抑制肝糖原分解和促进肝糖原合成。
Objective: To study the anti-hyperglycemic effect and its mechanism of Dendrobium candidum (DC). Method: Normal mice,adrenaline-induced hyperglycemic mice,streptozotocin-induced diabetic(STZ-DM) rats were used. The mechanisms of the anti-hyperglycemic action were studied with radio-immunoassay,immunohistochemical HRP-SPA stain,etc. Result: DC could not obviously decrease the serum glucose concentrations and insulin levels in normal mice. It could increase serum insulin levels and decrease serum glucagons concentrations in STZ-DM rats. The results of immunohistochemical stain demonstrated that the number of islet βcells was increased and that of islet α cells was decreased in STZ-DM rats. It could also decrease the serum glucose concentrations and increase liver glucogen contents in adrenaline-induced hyperglycemic mice. Conclusion: DC has obvious anti-hyperglycemic effects in adrenaline-induced hyperglycemic mice and STZ-DM rats. Its mechanisms are stimulating the secretion of insulin from β cells and inhibiting the secretion of glucagons from α cells,and it can probably decrease the decomposition of liver glucogen and increase the synthesis of liver glucogen.

关键词(KeyWords): 铁皮石斛;链脲佐菌素;血糖;胰岛素;胰高血糖素
Dendrobium candidum ;streptozotocin;blood sugar;insulin;glucagon

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作者(Author): 吴昊姝,徐建华,陈立钻,孙继军
WU Hao-shu 1,XU Jian-hua 1,CHEN Li-zuan 2,SUN Ji-jun 2 ( 1.Department of Pharmacology and Toxicology,Collage of Pharmaceutical Sciences,Zhejiang University,Hangzhou 310031,China; 2.Zhejiang TianHuang Pharmaceutical CO.LTD. Hangzhou 310012,China )

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