中国中药杂志

2014, v.39(21) 4240-4245

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穿心莲UPLC-PDA指纹图谱研究及4种内酯类成分含量测定
Research on UPLC-PDA fingerprint of andrographis paniculata and quantitative determination of 4 major constituents

黄竞怡;刘小琳;周水平;佟玲;丁黎;
HUANG Jing-yi;LIU Xiao-lin;ZHOU Shui-ping;TONG Ling;DING Li;Department of Pharmaceutical Analysis,China Pharmaceutical University;Tasly Pharmaceutical Analysis Institute,Tianjin Tasly Group Co. ,Ltd.;

摘要(Abstract):

分析研究不同部位及产地穿心莲药材,为穿心莲相关制剂工艺提供依据。以穿心莲内酯峰为参照,确定了27个共有指纹峰,建立了23批穿心莲药材的UPLC-PDA数字化定量指纹图谱,特征提取后对其进行主成分分析(PCA);采用外标法测定23批穿心莲药材中穿心莲内酯、新穿心莲内酯、去氧穿心莲内酯、脱水穿心莲内酯成分含量,并采用Plackett-Burman试验设计结合pareto图研究耐用性因素对方法的影响。结果发现,穿心莲药材部位比产地对药材质量的影响更显著;药材不同部位4种内酯含量差异大;流动相p H是影响方法耐用性较大的因素。
Andrographis paniculata from different parts and origins were analyzed by UPLC-PDA fingerprint to provide reference for related preparation technology. Using the peak of andrographolide as reference,27 common peaks were identified,and digitized UPLC-PDA fingerprints for 23 batches of andrographis paniculata were established in this research. Principal component analysis(PCA) was carried out after feature extraction. The contents of andrographolide,neoandrographolide,deoxyandrographolide,dehydroandrographolide were determined by external standard method. The Plackett-Burman design combined with pareto chart was used to analyze the factors influencing the robustness of the method. It was found that the medicinal part has a more remarkable influence on the quality of andrographis paniculata than the origin. The contents of the 4 lactones the differ greatly in the different parts of andrographis paniculata,and the p H of the mobile phase is an important factor that influenced the robustness of the method.

关键词(KeyWords): 穿心莲;指纹图谱;含量测定;PCA;Plackett-Burman设计
andrographis paniculata;fingerprint;quantitative determination;PCA;Plackett-Burman design

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基金项目(Foundation): 国家“重大新药创制”科技重大专项(2013ZX09402202)

作者(Author): 黄竞怡;刘小琳;周水平;佟玲;丁黎;
HUANG Jing-yi;LIU Xiao-lin;ZHOU Shui-ping;TONG Ling;DING Li;Department of Pharmaceutical Analysis,China Pharmaceutical University;Tasly Pharmaceutical Analysis Institute,Tianjin Tasly Group Co. ,Ltd.;

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