中国中药杂志

2011, v.36(07) 868-870

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HPLC同时测定天钩胶囊中4种有效成分的含量
Simultaneous determination of four active compounds in Tiangou capsules by HPLC

王信;李凌军;代龙;
WANG Xin,LI Lingjun,DAI Long(Shandong University of Traditional Chinese Medicine,Jinan 250355,China)

摘要(Abstract):

目的:建立同时测定天钩胶囊中天麻素、黄芩苷、丹皮酚、松脂醇二葡萄糖苷的HPLC方法。方法:采用Sino-Chrom ODS-BP柱(4.6 mm×150 mm,5μm),流动相乙腈-0.2%磷酸水溶液梯度洗脱,检测波长230 nm,流速1.0 mL.min-1,柱温30℃。结果:天麻素、黄芩苷、丹皮酚、松脂醇二葡萄糖苷分别在0.116 8~1.168 0(r=0.999 7),0.114 2~1.142 0(r=0.999 4),0.051 2~0.512 0(r=0.999 8),0.055 6~0.556 0μg(r=0.999 5)线性关系良好,平均加样回收率分别为100.3%(RSD 0.53%),99.96%(RSD 1.2%),99.90%(RSD 1.2%),99.97%(RSD 1.6%)。结论:所建立的方法简单、准确、可靠,重复性好,可用于天钩胶囊的质量控制。
Objective: To establish a method for quantitative analysis of gastrodin,baicalin,paeonolum and pinoresinol diglucoside in Tiangou capsules by HPLC.Method: The separation was performed on SinoChrom ODS-BP column(4.6 mm×150 mm,5 μm).The mobile phase consisted of acetonitrile and 0.2% phosphoric acid aqueous solution with gradient elution program.The flow rate was 1.0 ml·min-1 and the column temperature was 30 ℃.UV detection wavelength was set at 230 nm.Result: The linear ranges of gastrodin,baicalin,paeonolum and pinoresinol diglucoside were 0.116 8-1.168 0,0.114 2-1.142 0,0.051 2-0.512 0,0.055 6-0.556 0 μg,respectively.The average recoveries of gastrodin,baicalin,paeonolum and pinoresinol diglucoside were 100.3%(RSD 0.53%),99.96%(RSD 1.15%),99.90%(RSD 1.17%),99.97%(RSD 1.62%),respectively.Conclusion: The method is accurate,simple,feasible and reliable,and is available for the quality control of Tiangou capsules.

关键词(KeyWords): 天钩胶囊;天麻素;黄芩苷;丹皮酚;松脂醇二葡萄糖苷;HPLC
Tiangou capsules;gastrodin;baicalin;paeonolum;pinoresinol diglucoside;HPLC

Abstract:

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基金项目(Foundation): 国家“重大新药创制”科技重大专项(2009ZX09103-403)

作者(Author): 王信;李凌军;代龙;
WANG Xin,LI Lingjun,DAI Long(Shandong University of Traditional Chinese Medicine,Jinan 250355,China)

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