中国中药杂志

2009, v.34(17) 2177-2180

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板蓝根切制工艺的优选及切制前后指纹图谱的变化研究
Optimizing cutting process of Radix Isatidis and comparing difference of fingerprints before and after cutting

李友;马莉;龚慕辛;关怀;
LI You,MA Li*,GONG Muxin,GUAN Huai(College of Traditional Chinese Medicine,Capital Medical University,Beijing 100069,China)

摘要(Abstract):

目的:优选板蓝根软化切制的工艺条件;通过比较不同产地板蓝根药材切制前后指纹图谱的差异性,客观反应润药切制对药材总体成分的影响。方法:以药材软化时加水量、润制时间和饮片切制厚度为考察因素,以水溶性浸出物为指标,采用正交设计,优选板蓝根软化切制的条件;采用反相高效液相色谱法,Zorbax SB-C18(4.6 mm×250 mm,5μm)柱,流动相乙腈-水,梯度洗脱,检测波长238 nm,进样量10μL。对板蓝根粗粉和水润切制后的饮片进行HPLC测定,并采用中药色谱指纹图谱相似度评价软件进行比较。结果:板蓝根软化切制的最佳工艺为:0.6倍量的水,浸润24 h,饮片厚度为2 mm;结论:建立了板蓝根药材醋酸乙酯萃取物HPLC指纹图谱。板蓝根在切制前后,指纹图谱对照谱图差异明显,切制后中等极性部分损失较多。
Objective: For optimizing the cutting process of Radix Isatidis,the influence of cutting process on the whole chemical constituents was studied by comparing the difference of HPLC fingerprints of Radix Isatidis from various sources before and after cutting.Method: L9(34) orthogonal table were designed with three factors: The amount of solvent,soaking time and thickness of pieces.According to the amount of water-soluble extraction,orthogonal design was applied to processing;Fingerprints of acetoacetate extraction from 9 batches of Radix Isatidis from various sources before and after cutting were established with a High Performance Liquid Chromatography method based on a Agilent Zorbax SB-C18 column(4.6 mm×250 mm,5 μm) with gradient elution solvent system composed of acetonitrile-water solution;the detection wave length was set at 238 nm;sample injection was 10 μL.The fingerprints were compared with similarity evaluation software published by the Committee of China Codex.Result: The best process of cutting was following: the amount of solvent was 0.6 times of amount of Radix Isatidis,soaked for 24 h and the thickness of piece was 2 mm.Conclusion: The obvious difference is displayed by the comparing of HPLC fingerprints.It is indicated that the constituents in polarity medium lost a lot after cutting process.

关键词(KeyWords): 板蓝根;切制工艺;正交设计;指纹图谱
Radix Isatidis;cutting process;orthogonal design;fingerprint

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(30701091);; 北京市优秀人才培养项目(20071D0501800248)

作者(Author): 李友;马莉;龚慕辛;关怀;
LI You,MA Li*,GONG Muxin,GUAN Huai(College of Traditional Chinese Medicine,Capital Medical University,Beijing 100069,China)

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