中国中药杂志

2014, v.39(20) 3991-4000

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基于HPLC-DAD指纹图谱的远志质量评价研究
Study on quality assessment of Polygalae Radix based on HPLC-DAD fingerprint

赵云生;刘秀;毛福英;田洪岭;万德光;
ZHAO Yun-sheng;LIU Xiu;MAO Fu-ying;TIAN Hong-ling;WAN De-guang;Ningxia Medical University Pharmacy College;Ningxia Research Center of Modern Hui Medicine Engineering and Technology;Industrial Crop Research Institute,Shanxi Academy of Agricultural Sciences;Chengdu University of Traditional Chinese Medicine;

摘要(Abstract):

目的:建立远志HPLC指纹图谱,评价远志药材、远志根心与不同采收或生长时间远志质量。方法:采用Kromasil C18色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈和0.05%磷酸水溶液,梯度洗脱,流速1.0 m L·min-1,检测波长314 nm,柱温30℃。采用相似度计算、聚类分析、主成分分析和神经网络模型对远志药材质量进行判别分析。结果:26批不同产地远志药材中,相似度大于0.8的有24批;5批不同采收月份或生长时期的栽培远志药材中,相似度大于0.8的有4批;8批远志根心相似度均大于0.875。聚类分析、主成分分析和神经网络模型对远志药材质量判别分析结果与相似度分析结果一致。结论:指纹图谱结合化学模式识别技术可以有效评价远志质量,在栽培远志、野生远志、不同采收月份或生长时期的远志与远志根心中,有效物质种类均相似,但各色谱峰面积不同,远志野生变家栽后,有效物质群含量与野生药材相似,远志根心各色谱峰峰面积远小于远志药材,远志有效物质群积累主要与采收月份有关,与生长月数关系不明显。
Objective: To establish an HPLC fingerprint to evaluate the quality of Polygalae Radix,root xylem,and those collected in different growth ages or harvest time. Method: Separation was performed at 30 ℃ on a Kromasil C18column( 4. 6 mm × 250 mm,5 μm); the mobile phases was acetonitrile and 0. 05% H3PO4 water in the gradient elution; the flow rate was set at 1. 0 m L·min- 1and the detection wavelength at 314 nm; the quality discriminant analyses were accomplished by means of similarity analysis,cluster analysis,principal component analysis and neural network model. Result: In 26 batches of Polygalae Radix,24 batches fingerprint similarities were above 0. 8. In 5 different growth or harvest time batches,4 batches were above 0. 8; in 8 batches root xylem samples,the similarities were all above 0. 875. The similarity analysis was in accord with the quality discriminant analysis of cluster analysis,principal component analysis and neural network model. Conclusion: Fingerprint combined with chemical pattern recognition technique can effectively evaluate the quality of Polygalae Radix. The active substance species are all similar in cultivated,wild,different growth or harvest time Polygalae Radix and polygala root xylem,but the chromatography peak areas are different. The effective material contents are similar between wild and cultivated Polygalae Radix,but each chromatographic peak area of the root xylem is much smaller than that of Polygalae Radix. The chemical substance accumulation mainly depends on harvest month,but little growth time in Polygalae Radix.

关键词(KeyWords): 远志;指纹图谱;质量评价;模式识别
Polygalae Radix;fingerprint;quality evaluation;pattern recognition

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基金项目(Foundation): 国家自然科学基金项目(31270381)

作者(Author): 赵云生;刘秀;毛福英;田洪岭;万德光;
ZHAO Yun-sheng;LIU Xiu;MAO Fu-ying;TIAN Hong-ling;WAN De-guang;Ningxia Medical University Pharmacy College;Ningxia Research Center of Modern Hui Medicine Engineering and Technology;Industrial Crop Research Institute,Shanxi Academy of Agricultural Sciences;Chengdu University of Traditional Chinese Medicine;

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