中国中药杂志

2014, v.39(07) 1173-1178

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藏药余甘子药材及其鞣质部位的指纹图谱评价研究
HPLC-fingerprint-based quality evaluation on a Tibetan medicine Phyllanthus emblica and its tannin parts

孙雪飞;张鸿雁;夏青;赵海娟;吴玲芳;张兰珍;石任兵;
SUN Xue-fei;ZHANG Hong-yan;XIA-qing;ZHAO Hai-juan;WU Ling-fang;ZHANG Lan-zhen;SHI Ren-bing;School of Chinese Materia Medica,Beijing University of Chinese Medicine;

摘要(Abstract):

建立不同产地藏药余甘子药材及其鞣质部位HPLC指纹图谱。采用Diamonsil C18(4.6 mm×250 mm,5μm)色谱柱,甲醇-0.2%冰醋酸水溶液为流动相梯度洗脱,流速1 mL·min-1,柱温30℃,检测波长260 nm。提取13个色谱峰为药材指纹图谱共有峰,11个色谱峰为鞣质部位指纹图谱共有峰,采用对照品指认了5个主要色谱峰,运用相似度评价软件对所收集的8个不同产地藏药余甘子药材及其鞣质部位进行系统比较与归类,8个余甘子药材相似度在0.763~0.993,鞣质部位相似度在0.903~0.991,通过聚类分析、主成分分析将不同产地的余甘子药材和鞣质部位分别聚为3类。该法重复性好,简便可靠,为不同产地藏药余甘子药材及其鞣质部位质量控制和评价提供依据。
This study is to establish the fingerprint for Phyllanthus emblica and their tannin parts from different habitats by HPLC for its quality control. The determination was carried out on a Diamonsil C18( 4. 6 mm ×250 mm,5 μm) column,with methanol-0. 2% glacial acetic acid as mobile phase with gradient elution at a flow rate of 1 mL·min-1. The temperature was maintained at 30 ℃ and the detected wavelength is 260 nm,Thirteen chromatographic peaks were extracted as the common peaks of the fingerprint of P. emblica,and eleven as the common peaks of P. emblica tannin parts,and five peaks were identified by comparing with referent samples. The fingerprints of 8 samples were compared and classified by similarity evaluation,cluster analysis and principal component analysis( PCA). The similarity degrees of eight P. emblica were between 0. 763 and 0. 993,while tannin parts were between 0. 903 and 0. 991. All the samples of P. emblica and their tannin parts were classified into 3 categories. The method was so highly reproducible,simple and reliable that it could provide basis for quality control and evaluation of P. emblica from different habitats.

关键词(KeyWords): 藏药;余甘子;鞣质部位;HPLC指纹图谱;质量评价
Tibetan medicine;Phyllanthus emblica;tannin parts;HPLC-fingerprint;quality evaluation

Abstract:

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基金项目(Foundation): 国家自然科学基金项目(81274187);; 北京中医药大学科研创新团队项目(2011-CXTD-12);; 北京中医药大学自主课题(2009-TYB22-JS-027)

作者(Author): 孙雪飞;张鸿雁;夏青;赵海娟;吴玲芳;张兰珍;石任兵;
SUN Xue-fei;ZHANG Hong-yan;XIA-qing;ZHAO Hai-juan;WU Ling-fang;ZHANG Lan-zhen;SHI Ren-bing;School of Chinese Materia Medica,Beijing University of Chinese Medicine;

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