中国中药杂志

2011, v.36(06) 718-720

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桃仁药材UPLC特征指纹图谱研究
UPLC characteristic chromatographic profile of Persicae Semen

沈旭;李清;王振中;萧伟;罗杰;毕开顺;贾英;
SHEN Xu1,LI Qing2,WANG Zhenzhong3,XIAO Wei3,LUO Jie1,BI Kaishun2,JIA Ying1*(1.School of Traditional Chinese Materia Medica,Shenyang Pharmaceutical University,Shenyang 110016,China; 2.School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China; 3.Jiangsu Kanion Pharmaceutical Co.,Ltd.,Lianyungang 222001,China)

摘要(Abstract):

目的:建立桃仁药材的超高效液相特征性指纹图谱的测定方法,为有效控制和科学评价桃仁药材质量提供依据。方法:采用ACQUITY UPLC HSS T3色谱柱(2.1 mm×100 mm,1.8μm);流动相乙腈-0.05%磷酸水溶液,梯度洗脱;检测波长254 nm。对15批桃仁药材进行特征指纹图谱分析。结果:首次建立了桃仁药材的UPLC特征指纹图谱共有模式,共标定了12个共有峰,指认了其中6个共有峰,15批桃仁药材的相似度为0.884~0.996。结论:本方法快速、高效,可用于桃仁药材的质量评价。
Objective: To develop an UPLC method of determining the characteristic chromatographic profile of Persicae Semen for controlling the drug quality quickly and accurately.Method: The UPLC characteristic chromatographic profiles of fifteen batches of Persicae Semen were determined on an HSS T3 column(2.1 mm×100 mm,1.8 μm) eluted with the mobile phase consisted of acetonitrile-water containing 0.05% phosphoric acid in gradient mode and the detection wavelength was at 254 nm.Result: The common mode of the UPLC characteristic chromatographic profile was set up.There were 12 common peaks in the fingerprints of fifteen samples,six of which were identified,and the similar degrees of the fifteen batches to the common mode were between 0.884-0.996.Conclusion: The method was quick and accurate.The characteristic chromatographic profile of Persicae Semen with high specificity can be used to control the quality of Persicae Semen.

关键词(KeyWords): 桃仁;特征指纹图谱;UPLC
Persicae Semen;characteristic chromatographic profile;UPLC

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基金项目(Foundation): 国家“重大新药创制”科技重大专项(2009ZX09308-003,2009ZX09504-004)

作者(Author): 沈旭;李清;王振中;萧伟;罗杰;毕开顺;贾英;
SHEN Xu1,LI Qing2,WANG Zhenzhong3,XIAO Wei3,LUO Jie1,BI Kaishun2,JIA Ying1*(1.School of Traditional Chinese Materia Medica,Shenyang Pharmaceutical University,Shenyang 110016,China; 2.School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China; 3.Jiangsu Kanion Pharmaceutical Co.,Ltd.,Lianyungang 222001,China)

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