中国中药杂志

2010, v.35(06) 729-731

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高效液相色谱法测定酒豨莶草中奇壬醇的含量
Determination of kirenol in Herba Siegesbeckiae Preparata by high performance liquid chromatography

刘赞;侴桂新;王峥涛;
LIU Zan1,CHOU Guixin2*,WANG Zhengtao1,2*(1.China Pharmaceutical University,Nanjing 210038,China;2.Shanghai Research and Development Center for Standerdization of Chinese Medicines,Shanghai 201203,China)

摘要(Abstract):

目的:建立测定酒豨莶草中奇壬醇含量的高效液相色谱方法。方法:Boston Crest ODS色谱柱(4.6 mm×250mm,5μm);以乙腈为流动相A,以水为流动相B,进行梯度洗脱;检测波长215 nm,流速1.0 mL.min-1。结果:奇壬醇在0.020 220.02μg呈良好的线性关系(r=0.999 8),该方法平均回收率为99.62%,RSD 2.0%。结论:该方法简便易行、准确、重复性好,可用于酒豨莶草中奇壬醇的含量测定。
Objective:To establish a high performance liquid chromatographic method for the determination of kirenol in Herba Siegesbeckiae Praeparata.Method: The analysis was carried out on a Boston Crest ODS column(4.6 mm×250 mm,5 μm) with gradient elution using acetonitrile-water as mobile phases.The flow rate was 1.0 mL·min-1 and the detection wavelength was at 215 nm.Result: The calibration curve was linear over the range of 0.020 2-20.02 μg for kirenol.The correlation coefficient of the calibration curve was 0.999 75.The average recovery was 99.62% with relative standard derivation(RSD) of 2.0%.Conclusion: The results showed that the method is simple,accurate and repeatable and it is suitable for the determination of kirenol in Herba Siegesbeckiae Praeparata.

关键词(KeyWords): 酒豨莶草;奇壬醇;高效液相色谱法;含量测定
Herba Siegesbeckiae Preparata;kirenol;HPLC;determination

Abstract:

Keywords:

基金项目(Foundation): 《中国药典》2010年版一部标准研究课题任务书(YS-178);; 上海市科学技术委员会中药现代化专项课题(08DZ1970103)

作者(Authors): 刘赞;侴桂新;王峥涛;
LIU Zan1,CHOU Guixin2*,WANG Zhengtao1,2*(1.China Pharmaceutical University,Nanjing 210038,China;2.Shanghai Research and Development Center for Standerdization of Chinese Medicines,Shanghai 201203,China)

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